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Literature summary for 3.1.3.26 extracted from
- Purification and characterization of two phytases from Escherichia coli (1993), Arch. Biochem. Biophys., 303, 107-113.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| citrate | slight activation | Escherichia coli |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | slight | Escherichia coli | |
| Cu2+ | strong | Escherichia coli | |
| F- | - |
Escherichia coli | |
| Mn2+ | slight | Escherichia coli | |
| vanadate | - |
Escherichia coli | |
| Zn2+ | strong | Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.13 | - |
myo-inositol hexakisphosphate | - |
Escherichia coli | |
| 7.78 | - |
p-nitrophenyl phosphate | - |
Escherichia coli | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | 2 isoenzymes: P1 and P2 | Escherichia coli | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
1 * 42000, SDS-PAGE | Escherichia coli |
| 43000 | - |
gel filtration | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | highest activity under anaerobic conditions, during the exponential growth no activity observed | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
K12 ATCC 33965 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 8016 | - |
- |
Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | highest activity under anaerobic conditions, during the exponential growth no activity observed | Escherichia coli | ? | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Escherichia coli | myo-inositol pentakisphosphate + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Escherichia coli | p-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 42000, SDS-PAGE | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | 1 h, no loss of activity | Escherichia coli |
| 60 | - |
1 h, 76% loss of activity | Escherichia coli |
| 70 | - |
1 h, complete loss of activity | Escherichia coli |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 490 | - |
p-nitrophenyl phosphate | - |
Escherichia coli | |
| 6210 | - |
myo-inositol hexakisphosphate | - |
Escherichia coli | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
hydrolysis of myo-inositol hexakisphosphate | Escherichia coli |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 5.7 | pH 3.0: about 30% of maximal activity, pH 5.7: about 70% of maximal activity, hydrolysis of myo-inositol hexakisphosphate | Escherichia coli |
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