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Literature summary for 3.1.3.26 extracted from
- Rational design-based engineering of a thermostable phytase by site-directed mutagenesis (2018), Mol. Biol. Rep., 45, 2053-2061 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene appA, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S392F | site-directed mutagenesis, three-dimensional structure analysis, modeling, and comparison to wild-type structure. The mutant phytase shows 12.8%, 9.6% and 10.3% higher phytase activity at pH 6, 7 and 8, respectively, compared to wild-type enzyme, and the mutant enzymes shows thermostability improvement of 74% and 78.4% at 80°C and 90°C, respectively, compared to wild-type | Escherichia coli |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01945 | - |
myo-inositol hexakisphosphate | pH 5.0, 37°C, recombinant wild-type enzyme, substrate is sodium phytate | Escherichia coli |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| periplasm | - |
Escherichia coli | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | Escherichia coli | - |
1D-myo-inositol 1,2,3,5,6-pentakisphosphate + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | P07102 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol hexakisphosphate + H2O | - |
Escherichia coli | 1D-myo-inositol 1,2,3,5,6-pentakisphosphate + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | strong affinity for sodium phytate as substrate | Escherichia coli | 1D-myo-inositol 1,2,3,5,6-pentakisphosphate + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AppA | - |
Escherichia coli |
| periplasmic AppA protein | UniProt | Escherichia coli |
| phytase | - |
Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
wild-type and mutant enzymes | Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 80 | activity range of wild-type and mutant enzyme, profile overview | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
wild-type enzyme | Escherichia coli |
| 6 | - |
mutant S392F enzyme | Escherichia coli |
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