Cloned (Comment) | Organism |
---|---|
gene phy, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Penicillium griseoroseum strain PG63 protoplasts from vectorpAN-52-1-phy. Transformant T73 contains the phy gene integrated in at least three sites of the genome and shows the highest, 5.1fold increase in phytase activity in comparison to the host strain | Penicillium chrysogenum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | the enzyme sequence contains a signal peptide and a signal peptide cleavage site | Penicillium chrysogenum | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | Penicillium chrysogenum | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | Penicillium chrysogenum CCT 1273 | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Penicillium chrysogenum | - |
- |
- |
Penicillium chrysogenum CCT 1273 | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the enzyme sequence harbors putative N-linked glycosylation sites | Penicillium chrysogenum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Penicillium chrysogenum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1350 | - |
native enzyme from Penicillium chrysogenum strain CCT 1273, pH 5.0, 50°C | Penicillium chrysogenum |
2860 | - |
recombinant enzyme from Penicillium griseoroseum strain T73, pH 5.0, 50°C | Penicillium chrysogenum |
Storage Stability | Organism |
---|---|
PHY produced by Penicllium griseoroseum strain T73 retains 75%, 66% and 68% of the initial activity after four weeks of storage at -20°C, 8°C, and 25°C, respectively | Penicillium chrysogenum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme catalyzes phosphate release from cereals such as corn, soybean, and wheat | Penicillium chrysogenum | ? | - |
? | |
additional information | the enzyme catalyzes phosphate release from cereals such as corn, soybean, and wheat | Penicillium chrysogenum CCT 1273 | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Penicillium chrysogenum | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Penicillium chrysogenum CCT 1273 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 3.1.3.8 | Penicillium chrysogenum |
Phy | - |
Penicillium chrysogenum |
phytase | - |
Penicillium chrysogenum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Penicillium chrysogenum |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 70 | activity range, recombinant enzyme, maximal activity at 50°C, 64% at 40°C | Penicillium chrysogenum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Penicillium chrysogenum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 6 | maximal activity at pH 5.0 followed by pH 2.0 (73% of maximal activity), no activity at pH 7.0-8.0 | Penicillium chrysogenum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 8 | purified recombinant enzyme, 2 h at room temperature, completely stable | Penicillium chrysogenum |
General Information | Comment | Organism |
---|---|---|
evolution | the consensus sequence RHGXRXP and HD, typical of histidine acid phosphatases | Penicillium chrysogenum |