Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Burkholderia sp. a13(2014) | |
dithiothreitol | - |
Burkholderia sp. a13(2014) | |
EDTA | - |
Burkholderia sp. a13(2014) | |
EGTA | - |
Burkholderia sp. a13(2014) | |
additional information | no effect by PMSF at 1 mM | Burkholderia sp. a13(2014) |
Cloned (Comment) | Organism |
---|---|
gene phyA, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression in Escherichia coli strain HMS174 (DE3), subcloning in Escherichia coli strain JM109 | Burkholderia sp. a13(2014) |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Al3+ | 34% inhibition at 1 mM | Burkholderia sp. a13(2014) | |
Co2+ | 4.5% inhibition at 1 mM | Burkholderia sp. a13(2014) | |
Cu2+ | almost complete inhibition at 1 mM | Burkholderia sp. a13(2014) | |
Fe2+ | 62% inhibition at 1 mM | Burkholderia sp. a13(2014) | |
Fe3+ | 67.5% inhibition at 1 mM | Burkholderia sp. a13(2014) | |
Hg2+ | almost complete inhibition at 1 mM | Burkholderia sp. a13(2014) | |
iodoacetic acid | 70% inhibition at 1 mM | Burkholderia sp. a13(2014) | |
additional information | no effect by PMSF at 1 mM | Burkholderia sp. a13(2014) | |
Ni2+ | 17% inhibition at 1 mM | Burkholderia sp. a13(2014) | |
Pb2+ | 70% inhibition at 1 mM | Burkholderia sp. a13(2014) | |
Zn2+ | almost complete inhibition at 1 mM | Burkholderia sp. a13(2014) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.42 | - |
myo-inositol hexakisphosphate | pH 4.5, 50°C | Burkholderia sp. a13(2014) |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 10% activation at 1 mM | Burkholderia sp. a13(2014) | |
K+ | slight activation at 1 mM | Burkholderia sp. a13(2014) | |
Li+ | slight activation at 1 mM | Burkholderia sp. a13(2014) | |
Mn2+ | slight activation at 1 mM | Burkholderia sp. a13(2014) | |
additional information | poor effect by Mg2+ and Sr2+ at 1 mM | Burkholderia sp. a13(2014) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
gel filtration | Burkholderia sp. a13(2014) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | Burkholderia sp. a13(2014) | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | Burkholderia sp. a13(2014) JCM 30421 | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia sp. a13(2014) | A0A0E3VIU5 | isolated from Lake Kasumigaura, Japan | - |
Burkholderia sp. a13(2014) JCM 30421 | A0A0E3VIU5 | isolated from Lake Kasumigaura, Japan | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | enrichment cultivation using minimum medium containing phytic acid as the sole phosphorus source. Phytase production by strain a13 is induced by the presence of phytic acid and repressed by the addition of glucose | Burkholderia sp. a13(2014) | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
174.1 | - |
purified enzyme, pH 4.5, 50°C | Burkholderia sp. a13(2014) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme shows broad substrate specificity, but the highest activity is observed with phytic acid. Other substrates are 4-nitrophenyl phosphate, ATP, glucose-6-phosphate, and glycerol-3-phosphate. Phosphate detection by ammonium heptamolybdate reagent | Burkholderia sp. a13(2014) | ? | - |
? | |
additional information | the enzyme shows broad substrate specificity, but the highest activity is observed with phytic acid. Other substrates are 4-nitrophenyl phosphate, ATP, glucose-6-phosphate, and glycerol-3-phosphate. Phosphate detection by ammonium heptamolybdate reagent | Burkholderia sp. a13(2014) JCM 30421 | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Burkholderia sp. a13(2014) | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Burkholderia sp. a13(2014) JCM 30421 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 43000, SDS-PAGE, 1 * 46000, about, sequence calculation | Burkholderia sp. a13(2014) |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 3.1.3.8 | Burkholderia sp. a13(2014) |
PhyA | - |
Burkholderia sp. a13(2014) |
phytase | - |
Burkholderia sp. a13(2014) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | 55 | - |
Burkholderia sp. a13(2014) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 60 | activity range, profile overview | Burkholderia sp. a13(2014) |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
236.2 | - |
myo-inositol hexakisphosphate | pH 4.5, 50°C | Burkholderia sp. a13(2014) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
- |
Burkholderia sp. a13(2014) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 6.5 | activity range, profile overview | Burkholderia sp. a13(2014) |
Organism | Comment | Expression |
---|---|---|
Burkholderia sp. a13(2014) | phytase production by strain a13 is repressed by the addition of glucose | down |
Burkholderia sp. a13(2014) | phytase production by strain a13 is induced by the presence of phytic acid | up |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme sequence contains histidine acid phosphatase (HAP) motifs (RHGXRXP and HD), indicating the classification of this enzyme in the HAP phytase family | Burkholderia sp. a13(2014) |
additional information | requirement of a thiol group for the activity | Burkholderia sp. a13(2014) |
physiological function | phytases are a special class of phosphatases that sequentially hydrolyse phytic acid to less-phosphorylated myo-inositol which release inorganic phosphates. This class of enzyme is widely distributed in nature and has been isolated from several sources, including plants, animals, and microorganisms | Burkholderia sp. a13(2014) |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
562.4 | - |
myo-inositol hexakisphosphate | pH 4.5, 50°C | Burkholderia sp. a13(2014) |