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Literature summary for 3.1.3.26 extracted from

  • Kim, B.H.; Lee, J.Y.; Lee, P.C.
    Purification, sequencing and evaluation of a divergent phytase from Penicillium oxalicum KCTC6440 (2015), J. Gen. Appl. Microbiol., 61, 117-123 .
    View publication on PubMed
Search for more literature for 3.1.3.26

Cloned(Commentary)

Cloned (Comment) Organism
gene phyA, DNA and amino acid sequence determination and analysis, sequence comparisons Penicillium oxalicum

Inhibitors

Inhibitors Comment Organism Structure
Al3+
-
 Penicillium oxalicum
Ba2+
-
 Penicillium oxalicum
Ca2+
-
 Penicillium oxalicum
Cd2+
-
 Penicillium oxalicum
Co2+
-
 Penicillium oxalicum
Cu2+
-
 Penicillium oxalicum
EDTA
-
 Penicillium oxalicum
Li+
-
 Penicillium oxalicum
Mg2+
-
 Penicillium oxalicum
Mn2+
-
 Penicillium oxalicum
additional information poor inhibition by Co2+ and Al3+ at 5 mM Penicillium oxalicum
Zn2+
-
 Penicillium oxalicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics and Lineweaver-Burk plots Penicillium oxalicum
0.48 0.545 myo-inositol hexakisphosphate pH 5.5, 55°C, sodium phytate Penicillium oxalicum

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Penicillium oxalicum
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol hexakisphosphate + H2O Penicillium oxalicum phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 1D-myo-inositol pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O Penicillium oxalicum KCTC6440 phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 1D-myo-inositol pentakisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Penicillium oxalicum D9I0I9
-
-
Penicillium oxalicum KCTC6440 D9I0I9
-
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme from culture medium 486.41fold by ammonium sulfate fractionation, cation and anion exchange chromatography, and dialysis Penicillium oxalicum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
447.5
-
 purified native enzyme, pH 5.5, 55°C Penicillium oxalicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme PhyA efficiently releases phosphate from feedstuffs such as soybean, rich bran, and corn meal. The enzyme shows significant substrate specificity for phytate and only low activity with 4-nitrophenyl phosphate, diphosphate, 2-naphthyl phosphate, alpha-glyceryl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, AMP, ADP, and ATP Penicillium oxalicum ?
-
 ?
additional information enzyme PhyA efficiently releases phosphate from feedstuffs such as soybean, rich bran, and corn meal. The enzyme shows significant substrate specificity for phytate and only low activity with 4-nitrophenyl phosphate, diphosphate, 2-naphthyl phosphate, alpha-glyceryl phosphate, beta-glycerylphosphate, glucose 1-phosphate, fructose 1-phosphate, fructose 6-phosphate, AMP, ADP, and ATP Penicillium oxalicum KCTC6440 ?
-
 ?
myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Penicillium oxalicum 1D-myo-inositol pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26, substrate is sodium phytate Penicillium oxalicum 1D-myo-inositol pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Penicillium oxalicum KCTC6440 1D-myo-inositol pentakisphosphate + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26, substrate is sodium phytate Penicillium oxalicum KCTC6440 1D-myo-inositol pentakisphosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Penicillium oxalicum

Synonyms

Synonyms Comment Organism
More cf. EC 3.1.3.8 Penicillium oxalicum
PhyA
-
 Penicillium oxalicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Penicillium oxalicum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 70 activity range, profile overview Penicillium oxalicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Penicillium oxalicum

pH Range

pH Minimum pH Maximum Comment Organism
3 7 activity range, profile overview. Over 70% of maximal activity at pH 4.5-6.5 Penicillium oxalicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 6.5 purified enzyme, stable at Penicillium oxalicum

General Information

General Information Comment Organism
additional information homology modeling and computational analysis of Penicillium oxalicum strain KCTC6440 PhyA, comparison to the structure of phytase PhyA from Penicillium oxalicum strain PJ3 Penicillium oxalicum