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Literature summary for 3.1.3.26 extracted from

  • Yu, P.; Wang, X.T.; Liu, J.W.
    Purification and characterization of a novel cold-adapted phytase from Rhodotorula mucilaginosa strain JMUY14 isolated from Antarctic (2015), J. Basic Microbiol., 55, 1029-1039 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
EDTA
-
Rhodotorula mucilaginosa
EGTA
-
Rhodotorula mucilaginosa

Application

Application Comment Organism
agriculture supplementation of phytases into the monogastric animals feed can reduce the phosphorus excretion and result in improved availability of trace elements, minerals, amino acids, and energy. The enzyme has great potential for feed applications, especially in aquaculture Rhodotorula mucilaginosa
nutrition due to its specific enzymatic activity, phytase is considered a green feed additive, which can effectively improve the availability of phytate-P and, simultaneously, eliminate the anti-nutritional function of phytate, resulting in a lower production cost and improved environmental protection Rhodotorula mucilaginosa

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic tree Rhodotorula mucilaginosa

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
Rhodotorula mucilaginosa
Cu2+
-
Rhodotorula mucilaginosa
Mn2+
-
Rhodotorula mucilaginosa
additional information the enzyme shows strong protease resistance, after being incubated with 0.1 mg/ml of pepsin and trypsin at 37°C for 1 h, the residual activity is 92.2% and 75.6%, respectively Rhodotorula mucilaginosa
phenylglyoxal hydrate
-
Rhodotorula mucilaginosa
PMSF
-
Rhodotorula mucilaginosa
SDS
-
Rhodotorula mucilaginosa
Zn2+
-
Rhodotorula mucilaginosa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Lineweaver-Burk plots Rhodotorula mucilaginosa
0.247
-
myo-inositol hexakisphosphate pH 5.0, 37°C Rhodotorula mucilaginosa

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme is secreted Rhodotorula mucilaginosa
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Rhodotorula mucilaginosa
Fe2+ activates Rhodotorula mucilaginosa
Fe3+ activates Rhodotorula mucilaginosa
K+ activates Rhodotorula mucilaginosa
Mg2+ activates Rhodotorula mucilaginosa
Na+ activates Rhodotorula mucilaginosa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol hexakisphosphate + H2O Rhodotorula mucilaginosa phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 1D-myo-inositol pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O Rhodotorula mucilaginosa JMUY14 phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 1D-myo-inositol pentakisphosphate + phosphate
-
?

Organism

Organism UniProt Comment Textmining
Rhodotorula mucilaginosa
-
isolated from Antarctic deep-sea sediment
-
Rhodotorula mucilaginosa JMUY14
-
isolated from Antarctic deep-sea sediment
-

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 15.2fold by ammonium sulfate fractionation, anion and cation exchange chromatography, and gel filtration, to homogeneity Rhodotorula mucilaginosa

Source Tissue

Source Tissue Comment Organism Textmining
cell culture fermentation is optimized by a response surface methodology based on the Box-Behnken design, production leads to maximum activity of phytase of 205.45 U/ml Rhodotorula mucilaginosa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
36.26
-
crude enzyme, pH 5.0, 37°C Rhodotorula mucilaginosa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information color reagents, containing 33.3% v/v nitric acid, 10% w/v ammonium molybdate, and 0.24% w/v ammonium vanadate v/v in a 2:1:1 ratio, for stop of enzyme reaction and product determination Rhodotorula mucilaginosa ?
-
?
additional information color reagents, containing 33.3% v/v nitric acid, 10% w/v ammonium molybdate, and 0.24% w/v ammonium vanadate v/v in a 2:1:1 ratio, for stop of enzyme reaction and product determination Rhodotorula mucilaginosa JMUY14 ?
-
?
myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Rhodotorula mucilaginosa 1D-myo-inositol pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O substrate is sodium phytate or calcium phytate, phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Rhodotorula mucilaginosa 1D-myo-inositol pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Rhodotorula mucilaginosa JMUY14 1D-myo-inositol pentakisphosphate + phosphate
-
?
myo-inositol hexakisphosphate + H2O substrate is sodium phytate or calcium phytate, phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Rhodotorula mucilaginosa JMUY14 1D-myo-inositol pentakisphosphate + phosphate
-
?

Subunits

Subunits Comment Organism
? x * 63000, about, sequence calculation Rhodotorula mucilaginosa

Synonyms

Synonyms Comment Organism
More cf. EC 3.1.3.8 Rhodotorula mucilaginosa
myo-inositol hexakisphosphate phosphohydrolase
-
Rhodotorula mucilaginosa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Rhodotorula mucilaginosa

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
additional information
-
a cold-adapted phytase Rhodotorula mucilaginosa
25 55 over 50% of maximal activity within this range, maximal activity at 50°C, inactive at 60°C Rhodotorula mucilaginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 75 the purified phytase retains 73.6% of its activity after being incubated at 50°C for 10 min, while its activity is quickly lost after incubation at 65°C or 70°C for 10 min Rhodotorula mucilaginosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Rhodotorula mucilaginosa

pH Range

pH Minimum pH Maximum Comment Organism
3 5.5 optimum pH 5.0, over 80% of maximal activity at pH 3.0-5.5, dramatical loss of activity above pH 5.5 Rhodotorula mucilaginosa

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 7 purified native enzyme is quite stable in this range Rhodotorula mucilaginosa

pI Value

Organism Comment pI Value Maximum pI Value
Rhodotorula mucilaginosa sequence calculation
-
4.33