Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Rhodotorula mucilaginosa | |
EGTA | - |
Rhodotorula mucilaginosa |
Application | Comment | Organism |
---|---|---|
agriculture | supplementation of phytases into the monogastric animals feed can reduce the phosphorus excretion and result in improved availability of trace elements, minerals, amino acids, and energy. The enzyme has great potential for feed applications, especially in aquaculture | Rhodotorula mucilaginosa |
nutrition | due to its specific enzymatic activity, phytase is considered a green feed additive, which can effectively improve the availability of phytate-P and, simultaneously, eliminate the anti-nutritional function of phytate, resulting in a lower production cost and improved environmental protection | Rhodotorula mucilaginosa |
Cloned (Comment) | Organism |
---|---|
phylogenetic tree | Rhodotorula mucilaginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Rhodotorula mucilaginosa | |
Cu2+ | - |
Rhodotorula mucilaginosa | |
Mn2+ | - |
Rhodotorula mucilaginosa | |
additional information | the enzyme shows strong protease resistance, after being incubated with 0.1 mg/ml of pepsin and trypsin at 37°C for 1 h, the residual activity is 92.2% and 75.6%, respectively | Rhodotorula mucilaginosa | |
phenylglyoxal hydrate | - |
Rhodotorula mucilaginosa | |
PMSF | - |
Rhodotorula mucilaginosa | |
SDS | - |
Rhodotorula mucilaginosa | |
Zn2+ | - |
Rhodotorula mucilaginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Lineweaver-Burk plots | Rhodotorula mucilaginosa | |
0.247 | - |
myo-inositol hexakisphosphate | pH 5.0, 37°C | Rhodotorula mucilaginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | the enzyme is secreted | Rhodotorula mucilaginosa | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | activates | Rhodotorula mucilaginosa | |
Fe2+ | activates | Rhodotorula mucilaginosa | |
Fe3+ | activates | Rhodotorula mucilaginosa | |
K+ | activates | Rhodotorula mucilaginosa | |
Mg2+ | activates | Rhodotorula mucilaginosa | |
Na+ | activates | Rhodotorula mucilaginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | Rhodotorula mucilaginosa | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | Rhodotorula mucilaginosa JMUY14 | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula mucilaginosa | - |
isolated from Antarctic deep-sea sediment | - |
Rhodotorula mucilaginosa JMUY14 | - |
isolated from Antarctic deep-sea sediment | - |
Purification (Comment) | Organism |
---|---|
native extracellular enzyme 15.2fold by ammonium sulfate fractionation, anion and cation exchange chromatography, and gel filtration, to homogeneity | Rhodotorula mucilaginosa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | fermentation is optimized by a response surface methodology based on the Box-Behnken design, production leads to maximum activity of phytase of 205.45 U/ml | Rhodotorula mucilaginosa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
36.26 | - |
crude enzyme, pH 5.0, 37°C | Rhodotorula mucilaginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | color reagents, containing 33.3% v/v nitric acid, 10% w/v ammonium molybdate, and 0.24% w/v ammonium vanadate v/v in a 2:1:1 ratio, for stop of enzyme reaction and product determination | Rhodotorula mucilaginosa | ? | - |
? | |
additional information | color reagents, containing 33.3% v/v nitric acid, 10% w/v ammonium molybdate, and 0.24% w/v ammonium vanadate v/v in a 2:1:1 ratio, for stop of enzyme reaction and product determination | Rhodotorula mucilaginosa JMUY14 | ? | - |
? | |
myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Rhodotorula mucilaginosa | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | substrate is sodium phytate or calcium phytate, phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Rhodotorula mucilaginosa | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Rhodotorula mucilaginosa JMUY14 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | substrate is sodium phytate or calcium phytate, phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 | Rhodotorula mucilaginosa JMUY14 | 1D-myo-inositol pentakisphosphate + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 63000, about, sequence calculation | Rhodotorula mucilaginosa |
Synonyms | Comment | Organism |
---|---|---|
More | cf. EC 3.1.3.8 | Rhodotorula mucilaginosa |
myo-inositol hexakisphosphate phosphohydrolase | - |
Rhodotorula mucilaginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Rhodotorula mucilaginosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
a cold-adapted phytase | Rhodotorula mucilaginosa |
25 | 55 | over 50% of maximal activity within this range, maximal activity at 50°C, inactive at 60°C | Rhodotorula mucilaginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | 75 | the purified phytase retains 73.6% of its activity after being incubated at 50°C for 10 min, while its activity is quickly lost after incubation at 65°C or 70°C for 10 min | Rhodotorula mucilaginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Rhodotorula mucilaginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 5.5 | optimum pH 5.0, over 80% of maximal activity at pH 3.0-5.5, dramatical loss of activity above pH 5.5 | Rhodotorula mucilaginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 7 | purified native enzyme is quite stable in this range | Rhodotorula mucilaginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rhodotorula mucilaginosa | sequence calculation | - |
4.33 |