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Literature summary for 3.1.3.26 extracted from

  • Chanderman, A.; Puri, A.; Permaul, K.; Singh, S.
    Production, characteristics and applications of phytase from a rhizosphere isolated Enterobacter sp. ACSS (2016), Bioprocess Biosyst. Eng., 39, 1577-1587 .
    View publication on PubMed
Search for more literature for 3.1.3.26

Application

Application Comment Organism
food industry the enzyme can be applied in dephytinizing animal feeds, and the baking industry. Effect of phytase supplementation in different doses on bread characteristics, overview Enterobacter sp. ACSS

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
 Enterobacter sp. ACSS
cetyltrimethylammoniumbromid CTAB Enterobacter sp. ACSS
Cu2+
-
 Enterobacter sp. ACSS
DTT
-
 Enterobacter sp. ACSS
EDTA
-
 Enterobacter sp. ACSS
Fe2+
-
 Enterobacter sp. ACSS
additional information the enzyme is fairly stable in the presence of pepsin under physiological conditions Enterobacter sp. ACSS
Pb2+
-
 Enterobacter sp. ACSS
SDS
-
 Enterobacter sp. ACSS
Triton X-100
-
 Enterobacter sp. ACSS
Tween 20
-
 Enterobacter sp. ACSS
Tween 80
-
 Enterobacter sp. ACSS
Zn2+
-
 Enterobacter sp. ACSS

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michealis-Menten kinetics Enterobacter sp. ACSS
0.21
-
 myo-inositol hexakisphosphate pH 2.5, 60°C Enterobacter sp. ACSS

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ activates Enterobacter sp. ACSS
Mg2+ activates Enterobacter sp. ACSS
Mn2+ activates Enterobacter sp. ACSS

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
-
 Enterobacter sp. ACSS

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
myo-inositol hexakisphosphate + H2O Enterobacter sp. ACSS phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 1D-myo-inositol pentakisphosphate + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Enterobacter sp. ACSS
-
 isolated from a rhizosphere
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 14.22fold by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration, to homogeneity Enterobacter sp. ACSS

Source Tissue

Source Tissue Comment Organism Textmining
additional information optimization of process parameters for phytase production by Enterobacter sp. ACSS leads to a 4.6fold improvement in submerged fermentation, which is enhanced further in fed-batch fermentation Enterobacter sp. ACSS
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
805.98
-
 purified enzyme, pH 2.5, 60°C Enterobacter sp. ACSS

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information hydrolysis of insoluble metal-phytates, overview. The enzyme is also active with 4-nitrophenyl phosphate, sodium diphosphate, glucose-1-phosphate, and glucose-6-phosphate Enterobacter sp. ACSS ?
-
 ?
myo-inositol hexakisphosphate + H2O phosphate cleavage position is not determined, cf. EC 3.1.3.8 and 3.1.3.26 Enterobacter sp. ACSS 1D-myo-inositol pentakisphosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 62000, SDS-PAGE Enterobacter sp. ACSS

Synonyms

Synonyms Comment Organism
More cf. EC 3.1.3.8 Enterobacter sp. ACSS
myo-inositolhexakisphosphate phosphohydrolase
-
 Enterobacter sp. ACSS
phytase
-
 Enterobacter sp. ACSS

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Enterobacter sp. ACSS

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 80 activity range Enterobacter sp. ACSS

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50 80 half-life of the enzyme at 50, 60, 70 and 80°C is 866.2, 693.0, 37.8 and 11.3 min, respectively. The activity is lost completely at 80°C after 55 min incubation Enterobacter sp. ACSS
80
-
 half-life is 11.3 min Enterobacter sp. ACSS

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1640
-
 myo-inositol hexakisphosphate pH 2.5, 60°C Enterobacter sp. ACSS

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5
-
-
 Enterobacter sp. ACSS

pH Range

pH Minimum pH Maximum Comment Organism
2 6 activity range Enterobacter sp. ACSS

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
 purified enzyme, retains complete activity for 120 min Enterobacter sp. ACSS
2 6 half-life of the enzyme at pH 2.0, 3.0, 4.0, 5.0 and 6.0 is 577.5, 433.1, 770.0, 247.5 and 91.2 min, respectively Enterobacter sp. ACSS
3 4 purified enzyme, loss of 10% activity after 120 min Enterobacter sp. ACSS
5
-
 purified enzyme, loss of 30% activity after 120 min Enterobacter sp. ACSS
6
-
 purified enzyme, loss of 60% activity after 120 min, and of all activity after 240 min Enterobacter sp. ACSS

General Information

General Information Comment Organism
physiological function phytase degrades phytates to release phosphate and other essential ions. It is widespread in nature, found in plants, animals and microorganisms. The use of phytase reduces phosphate excretion in monogastric animals and avoids supplementation of feeds with inorganic phosphate. Animal stomach is the main functional site for phytase-mediated dephosphorylation in the gastrointestinal tract Enterobacter sp. ACSS

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
7809.5
-
 myo-inositol hexakisphosphate pH 2.5, 60°C Enterobacter sp. ACSS