Any feedback?
Literature summary for 3.1.3.26 extracted from
- Cloning and characterization of a novel phytase from wastewater treatment yeast Hansenula fabianii J640 and expression in Pichia pastoris (2009), J. Biosci. Bioeng., 108, 225-230.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | 122% of initial activity | Cyberlindnera fabianii |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Cyberlindnera fabianii |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
x * 75000, native protein, x * 51000, deglycosylated protein, SDS-PAGE | Cyberlindnera fabianii |
| 75000 | - |
x * 75000, native protein, x * 51000, deglycosylated protein, SDS-PAGE | Cyberlindnera fabianii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cyberlindnera fabianii | C4B6T1 | - |
- |
| Cyberlindnera fabianii J640 | C4B6T1 | - |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | - |
Cyberlindnera fabianii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10.2 | - |
substrate D-glucose 1-phosphate, 37°C, pH 4.5 | Cyberlindnera fabianii |
| 22 | - |
substrate alpha-glycerophosphate, 37°C, pH 4.5 | Cyberlindnera fabianii |
| 31.6 | - |
substrate beta-glycerophosphate, 37°C, pH 4.5 | Cyberlindnera fabianii |
| 31.7 | - |
substrate D-glucose 6-phosphate, 37°C, pH 4.5 | Cyberlindnera fabianii |
| 46.1 | - |
substrate myo-inositol hexakisphosphate, 37°C, pH 4.5 | Cyberlindnera fabianii |
| 185 | - |
substrate 4-nitrophenyl phosphate, 37°C, pH 4.5 | Cyberlindnera fabianii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Cyberlindnera fabianii | 4-nitrophenol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Cyberlindnera fabianii J640 | 4-nitrophenol + phosphate | - |
? | |
| alpha-glycerophosphate + H2O | - |
Cyberlindnera fabianii | glycerol + phosphate | - |
? | |
| alpha-glycerophosphate + H2O | - |
Cyberlindnera fabianii J640 | glycerol + phosphate | - |
? | |
| beta-glycerophosphate + H2O | - |
Cyberlindnera fabianii | glycerol + phosphate | - |
? | |
| beta-glycerophosphate + H2O | - |
Cyberlindnera fabianii J640 | glycerol + phosphate | - |
? | |
| D-glucose 1-phosphate + H2O | - |
Cyberlindnera fabianii | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Cyberlindnera fabianii | D-glucose + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Cyberlindnera fabianii J640 | D-glucose + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Cyberlindnera fabianii | ? + phosphate | removal of 5 phosphate residues from each molecule of phytate | ? | |
| myo-inositol hexakisphosphate + H2O | - |
Cyberlindnera fabianii J640 | ? + phosphate | removal of 5 phosphate residues from each molecule of phytate | ? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 75000, native protein, x * 51000, deglycosylated protein, SDS-PAGE | Cyberlindnera fabianii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Cyberlindnera fabianii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Cyberlindnera fabianii |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
