Literature summary for 3.1.3.26 extracted from

Shao, N.; Huang, H.; Meng, K.; Luo, H.; Wang, Y.; Yang, P.; Yao, B.
Cloning, expression, and characterization of a new phytase from the phytopathogenic bacterium Pectobacterium wasabiae DSMZ 18074 (2008), J. Microbiol. Biotechnol., 18, 1221-1226.

Search for more literature for 3.1.3.26

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21	Pectobacterium wasabiae

Inhibitors

Inhibitors	Comment	Organism	Structure
Ag+	inhibits activity	Pectobacterium wasabiae
Co2+	inhibits activity	Pectobacterium wasabiae
Cr3+	inhibits activity	Pectobacterium wasabiae
Cu2+	inhibits activity	Pectobacterium wasabiae
Fe3+	inhibits activity	Pectobacterium wasabiae
Hg2+	inhibits activity	Pectobacterium wasabiae
Ni2+	inhibits activity	Pectobacterium wasabiae
Pd2+	inhibits activity	Pectobacterium wasabiae
SDS	inhibits activity	Pectobacterium wasabiae
Zn2+	inhibits activity	Pectobacterium wasabiae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.17	-	myo-inositol hexakisphosphate	Vmax: 1714 micromol/min/mg	Pectobacterium wasabiae

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Ca2+	slightly enhances activity	Pectobacterium wasabiae
EDTA	slightly enhances activity	Pectobacterium wasabiae
Mg2+	slightly enhances activity	Pectobacterium wasabiae
Na+	slightly enhances activity	Pectobacterium wasabiae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
45000	-	SDS-PAGE	Pectobacterium wasabiae
48000	-	predicted from cDNA	Pectobacterium wasabiae

Organism

Organism	UniProt	Comment	Textmining
Pectobacterium wasabiae	A0A1D7YZ01	The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate; DSMZ 18074, the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate	-

Purification (Commentary)

Purification (Comment)	Organism
using NH4SO4 precipitation and a HiTrap Q Sepharose XL FPLC column	Pectobacterium wasabiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
1072	-	activity of purified appA	Pectobacterium wasabiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
myo-inositol hexakisphosphate + H2O	The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate	Pectobacterium wasabiae	? + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
AppA	-	Pectobacterium wasabiae
phytase	-	Pectobacterium wasabiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Pectobacterium wasabiae
50	-	-	Pectobacterium wasabiae

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	enzyme retains stability when incubated at 50°C for 30 min, and over 70% activity retain. Enzyme loses activity rapidly at higher temperatures, with more than 90% activity lost after incubating at 70°C for 2 min	Pectobacterium wasabiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Pectobacterium wasabiae

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
3	10	activity is stable over a wide range of pH, more than 80% of the total activity is retained after incubation at pH 3.0-10.0 at 37°C for 60 min	Pectobacterium wasabiae

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)