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Literature summary for 3.1.3.26 extracted from

  • Singh, B.; Satyanarayana, T.
    Phytase production by Sporotrichum thermophile in a cost-effective cane molasses medium in submerged fermentation and its application in bread (2008), J. Appl. Microbiol., 105, 1858-1865.
    View publication on PubMed
Search for more literature for 3.1.3.26

Inhibitors

Inhibitors Comment Organism Structure
2,3-Butanedione
-
 Thermothelomyces heterothallicus
Al3+ inhibitory effect Thermothelomyces heterothallicus
Ba2+ slight inhibitory effect Thermothelomyces heterothallicus
Co2+ slight inhibitory effect Thermothelomyces heterothallicus
Cu2+ slight inhibitory effect Thermothelomyces heterothallicus
Fe2+ slight inhibitory effect Thermothelomyces heterothallicus
Fe3+ slight inhibitory effect Thermothelomyces heterothallicus
guanidinium hydrochloride
-
 Thermothelomyces heterothallicus
KI
-
 Thermothelomyces heterothallicus
Mg2+ slight stimulatory effect Thermothelomyces heterothallicus
Mn2+ slight inhibitory effect Thermothelomyces heterothallicus
Na+ slight inhibitory effect Thermothelomyces heterothallicus
Urea
-
 Thermothelomyces heterothallicus
Zn2+ slight inhibitory effect Thermothelomyces heterothallicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.156
-
 myo-inositol hexakisphosphate Vmax: 83 nmol/mg/s Thermothelomyces heterothallicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
90000
-
 SDS-PAGE Thermothelomyces heterothallicus
456000
-
 homopentamer, native PAGE Thermothelomyces heterothallicus

Organism

Organism UniProt Comment Textmining
Thermothelomyces heterothallicus
-
 the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein 14% carbohydrate Thermothelomyces heterothallicus

Purification (Commentary)

Purification (Comment) Organism
purified to homogeneity using acetone precipitation followed by ion-exchange and gel-filtration column chromatography Thermothelomyces heterothallicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Thermothelomyces heterothallicus 4-nitrophenol + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate Thermothelomyces heterothallicus ? + phosphate
-
 ?
phosphoenol pyruvate + H2O
-
 Thermothelomyces heterothallicus ?
-
 ?

Subunits

Subunits Comment Organism
homopentamer 4 * 90000, SDS-PAGE, gel filtration Thermothelomyces heterothallicus

Synonyms

Synonyms Comment Organism
phytase
-
 Thermothelomyces heterothallicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Thermothelomyces heterothallicus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 T1/2 of 16 h at 60°C and 1.5 h at 80°C Thermothelomyces heterothallicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Thermothelomyces heterothallicus