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Literature summary for 3.1.3.26 extracted from

  • Huang, H.; Luo, H.; Wang, Y.; Fu, D.; Shao, N.; Wang, G.; Yang, P.; Yao, B.
    A novel phytase from Yersinia rohdei with high phytate hydrolysis activity under low pH and strong pepsin conditions (2008), Appl. Microbiol. Biotechnol., 80, 417-426.
    View publication on PubMed
Search for more literature for 3.1.3.26

Application

Application Comment Organism
biotechnology Yersinia rohdei phytase is an attractive additive to animal feed Yersinia rohdei

Cloned(Commentary)

Cloned (Comment) Organism
recombinantly expressed in Pichia pastoris Yersinia rohdei
recombinantly expressed in Pichia pastoris Yersinia pestis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.196
-
 myo-inositol hexakisphosphate
-
 Yersinia pestis
0.206
-
 myo-inositol hexakisphosphate
-
 Yersinia rohdei

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45200
-
 SDS-PAGE Yersinia pestis
46100
-
 SDS-PAGE Yersinia rohdei

Organism

Organism UniProt Comment Textmining
Yersinia pestis Q7CIZ7
-
-
Yersinia rohdei B4X9S4
-
-

Purification (Commentary)

Purification (Comment) Organism
using ammonium sulfate precipitation and anion exchange chromatography Yersinia rohdei
using ammonium sulfate precipitation and anion exchange chromatography Yersinia pestis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 compared with the major commercial phytases from Aspergillus niger, Escherichia coli, and Yersinia intermedia, the Yersinia rohdei phytase is more resistant to pepsin, retains more activity under gastric conditions, and releases more inorganic phosphorus (two to ten times) from soybean meal under simulated gastric conditions Yersinia rohdei
2344
-
-
 Yersinia pestis
2456
-
-
 Yersinia rohdei

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol hexakisphosphate + H2O
-
 Yersinia rohdei ? + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O
-
 Yersinia pestis ? + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
4-phytase
-
 Yersinia rohdei
4-phytase
-
 Yersinia pestis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Yersinia rohdei
37
-
 assay at Yersinia pestis
55
-
-
 Yersinia rohdei
55
-
-
 Yersinia pestis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
80
-
 retains 14% activity at 80°C Yersinia pestis
80
-
 retains 50% activity at 80°C Yersinia rohdei

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2283
-
 myo-inositol hexakisphosphate
-
 Yersinia pestis
2531
-
 myo-inositol hexakisphosphate
-
 Yersinia rohdei

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Yersinia rohdei
4.5
-
-
 Yersinia pestis

pH Range

pH Minimum pH Maximum Comment Organism
2 4.5
-
 Yersinia rohdei
2 4.5
-
 Yersinia pestis

pH Stability

pH Stability pH Stability Maximum Comment Organism
1.5
-
 enzyme loses all its activity at pH: 1.5 Yersinia pestis
1.5 2.5 enzyme retains above 80% of activity after incubation at pH 2.5-8.0 for 60 min Yersinia rohdei
2.5 8 enzyme retains above 80% of activity after incubation at pH 2.5-8.0 for 60 min Yersinia rohdei
2.5 8 enzyme retains above 80% of activity after incubation at pH 2.5-8.0 for 60 min Yersinia pestis