Literature summary for 3.1.3.26 extracted from

Ragon, M.; Neugnot-Roux, V.; Chemardin, P.; Moulin, G.; Boze, H.
Molecular gene cloning and overexpression of the phytase from Debaryomyces castellii CBS 2923 (2008), Protein Expr. Purif., 58, 275-283.

Cloned(Commentary)

Cloned (Comment)	Organism
phytase is heterologously expressed in the methylotrophic yeast Pichia pastoris under both the Pichia pastoris inducible alcohol oxidase (AOX1) promoter and the constitutive glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter. Maximum production levels obtained are 476 U/ml, with the AOX1 expression system and 16.5 U/ml with the GAP one. These productions correspond to a 320fold and a 10fold overexpression of the protein, respectively, as compared to the homologous production	Debaryomyces castellii

Cloned (Comment)

Organism

phytase is heterologously expressed in the methylotrophic yeast Pichia pastoris under both the Pichia pastoris inducible alcohol oxidase (AOX1) promoter and the constitutive glyceraldehyde-3-phosphate dehydrogenase (GAP) promoter. Maximum production levels obtained are 476 U/ml, with the AOX1 expression system and 16.5 U/ml with the GAP one. These productions correspond to a 320fold and a 10fold overexpression of the protein, respectively, as compared to the homologous production

Debaryomyces castellii

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.24	-	myo-inositol hexakisphosphate	recombinant protein	Debaryomyces castellii
0.532	-	myo-inositol hexakisphosphate	native protein	Debaryomyces castellii

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
51200	-	predicted from cDNA	Debaryomyces castellii
53000	-	mass spectrometry	Debaryomyces castellii

Organism

Organism	UniProt	Comment	Textmining
Debaryomyces castellii	A2TBB4	the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
glycoprotein	-	Debaryomyces castellii

Purification (Commentary)

Purification (Comment)	Organism
using a HiPrep Phenyl FF column	Debaryomyces castellii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
101	-	crude extract, recombinant protein	Debaryomyces castellii
157.4	-	purified extract, native protein	Debaryomyces castellii
182	-	purified extract, recombinant protein	Debaryomyces castellii

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
myo-inositol hexakisphosphate + H2O	The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate	Debaryomyces castellii	? + phosphate	-	?
myo-inositol hexakisphosphate + H2O	The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate	Debaryomyces castellii CBS 2923	? + phosphate	-	?

Synonyms

Synonyms	Comment	Organism
phytase	-	Debaryomyces castellii

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Debaryomyces castellii
60	-	-	Debaryomyces castellii

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
66.5	-	thermic denaturation (1 h)	Debaryomyces castellii

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
51.2	-	myo-inositol hexakisphosphate	native protein	Debaryomyces castellii
59.2	-	myo-inositol hexakisphosphate	recombinant protein	Debaryomyces castellii

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4	-	optimum pH between 4.0-4.5	Debaryomyces castellii