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Literature summary for 3.1.3.26 extracted from

  • Garrett, J.B.; Kretz, K.A.; O'Donoghue, E.; Kerovuo, J.; Kim, W.; Barton, N.R.; Hazlewood, G.P.; Short, J.M.; Robertson, D.E.; Gray, K.A.
    Enhancing the thermal tolerance and gastric performance of a microbial phytase for use as a phosphate-mobilizing monogastric-feed supplement (2004), Appl. Environ. Microbiol., 70, 3041-3046.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.26

Protein Variants

Protein Variants Comment Organism
Q84W/Y277D/W68E/K97C/R181Y/N226C/A95P/S168E highly active phytase with no loss of activity after heating at 62°C for 1 h and 27% of its initial activity after 10 min at 85°C, which is a significant improvement over the appA parental phytase. The mutant enzyme also shows a 3.5fold enhancement in gastric stability Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Synonyms

Synonyms Comment Organism
appA phytase
-
 Escherichia coli

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
62
-
 1 h, no loss of activity, mutant enzyme Q84W/Y277D/W68E/K97C/R181Y/N226C/A95P/S168E Escherichia coli
64
-
 Tm-value for parental phytase is 63.7°C Escherichia coli
75.7
-
 Tm-value for mutant enzyme Q84W/Y277D/W68E/K97C/R181Y/N226C/A95P/S168E Escherichia coli
85
-
 10 min, 27% loss of activity,mutant enzyme Q84W/Y277D/W68E/K97C/R181Y/N226C/A95P/S168E Escherichia coli