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Literature summary for 3.1.3.26 extracted from
- Identification and characterization of a phytase of potential commercial interest (2004), J. Biotechnol., 110, 313-322.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| F- | activates | Rhizopus microsporus var. oligosporus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Rhizopus microsporus var. oligosporus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0104 | - |
myo-inositol hexakisphosphate | pH 5.5 | Rhizopus microsporus var. oligosporus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cd2+ | activates | Rhizopus microsporus var. oligosporus | |
| Hg2+ | activates | Rhizopus microsporus var. oligosporus | |
| Mg2+ | activates | Rhizopus microsporus var. oligosporus | |
| Zn2+ | activates | Rhizopus microsporus var. oligosporus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 120000 | - |
1 * 120000, SDS-PAGE | Rhizopus microsporus var. oligosporus |
| 124000 | - |
gel filtration | Rhizopus microsporus var. oligosporus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhizopus microsporus var. oligosporus | - |
ATCC 22959. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 30.5% total carbohydrate | Rhizopus microsporus var. oligosporus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhizopus microsporus var. oligosporus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Rhizopus microsporus var. oligosporus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-naphthyl phosphate + H2O | 89.6% of the activity with myo-inositol hexakisphosphate | Rhizopus microsporus var. oligosporus | 1-naphthol + phosphate | - |
? | |
| 2-naphthyl phosphate + H2O | 97.1% of the activity with myo-inositol hexakisphosphate | Rhizopus microsporus var. oligosporus | 2-naphthol + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | 59.6% of the activity with myo-inositol hexakisphosphate | Rhizopus microsporus var. oligosporus | 4-nitrophenol + phosphate | - |
? | |
| ADP + H2O | 14.2% of the activity with myo-inositol hexakisphosphate | Rhizopus microsporus var. oligosporus | ? | - |
? | |
| AMP + H2O | 25.0% of the activity with myo-inositol hexakisphosphate | Rhizopus microsporus var. oligosporus | adenosine + phosphate | - |
? | |
| ATP + H2O | 22.3% of the activity with myo-inositol hexakisphosphate | Rhizopus microsporus var. oligosporus | ? | - |
? | |
| D-glucose 1-phosphate + H2O | 92.0% of the activity with myo-inositol hexakisphosphate | Rhizopus microsporus var. oligosporus | D-glucose + phosphate | - |
? | |
| glycerol 2-phosphate + H2O | 85.6% of the activity with myo-inositol hexakisphosphate | Rhizopus microsporus var. oligosporus | glycerol + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | - |
Rhizopus microsporus var. oligosporus | ? + phosphate | - |
? | |
| phenyl phosphate + H2O | 89.2% of the activity with myo-inositol hexakisphosphate | Rhizopus microsporus var. oligosporus | phenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 120000, SDS-PAGE | Rhizopus microsporus var. oligosporus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
- |
Rhizopus microsporus var. oligosporus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | 75 | 37°C: about 65% of maximal activity, 75°C: about 45% of maximal activity | Rhizopus microsporus var. oligosporus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
about 25% loss of activity after 1 min, about 50% loss of activity after 2 min, about 65% loss of activity after 3 min | Rhizopus microsporus var. oligosporus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 51 | - |
myo-inositol hexakisphosphate | pH 5.5 | Rhizopus microsporus var. oligosporus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Rhizopus microsporus var. oligosporus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 1.8 | 6 | pH 1.8: about 45% of maximal activity, pH 6.0: about 45% of maximal activity | Rhizopus microsporus var. oligosporus |
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