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Literature summary for 3.1.3.26 extracted from

  • Greiner, R.
    Purification and characterization of three phytases from germinated lupine seeds (Lupinus albus var. amiga) (2002), J. Agric. Food Chem., 50, 6858-6864.
    View publication on PubMed
Search for more literature for 3.1.3.26

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 0.1 mM-1 mM, slight inhibition Lupinus albus
Cu2+ 0.1 mM-1 mM, strong inhibition Lupinus albus
F-
-
 Lupinus albus
Fe2+ 0.1 mM-1 mM, strong inhibition Lupinus albus
Fe3+ 0.1 mM-1 mM, strong inhibition Lupinus albus
Mn2+ 0.1 mM-1 mM, slight inhibition Lupinus albus
molybdate
-
 Lupinus albus
additional information Mg2+ and Ca2+ show no effect at 0.1 mM-1 mM Lupinus albus
phosphate
-
 Lupinus albus
vanadate
-
 Lupinus albus
wolframate
-
 Lupinus albus
Zn2+ 0.1 mM-1 mM, strong inhibition Lupinus albus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.08
-
 myo-inositol hexakisphosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
0.123
-
 4-nitrophenyl phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
0.13
-
 myo-inositol hexakisphosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
0.152
-
 4-nitrophenyl phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
0.176
-
 4-nitrophenyl phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
0.217
-
 GTP pH 5.0, 35°C, phytase LP2 Lupinus albus
0.229
-
 D-fructose 1,6-diphosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
0.3
-
 myo-inositol hexakisphosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
0.302
-
 D-glucose 6-phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
0.315
-
 AMP pH 5.0, 35°C, phytase LP12 Lupinus albus
0.319
-
 AMP pH 5.0, 35°C, phytase LP2 Lupinus albus
0.359
-
 D-fructose 1,6-diphosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
0.365
-
 AMP pH 5.0, 35°C, phytase LP11 Lupinus albus
0.398
-
 GTP pH 5.0, 35°C, phytase LP11 Lupinus albus
0.398
-
 ADP pH 5.0, 35°C, phytase LP12 Lupinus albus
0.401
-
 D-glucose 6-phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
0.403
-
 ADP pH 5.0, 35°C, phytase LP11 Lupinus albus
0.412
-
 ATP pH 5.0, 35°C, phytase LP12 Lupinus albus
0.423
-
 GTP pH 5.0, 35°C, phytase LP12 Lupinus albus
0.445
-
 diphosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
0.451
-
 ADP pH 5.0, 35°C, phytase LP2 Lupinus albus
0.478
-
 D-fructose 6-phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
0.49 2 1-naphthyl phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
0.512
-
 2-naphthyl phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
0.516
-
 1-naphthyl phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
0.531
-
 ATP pH 5.0, 35°C, phytase LP11 Lupinus albus
0.531
-
 D-fructose 6-phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
0.596
-
 ATP pH 5.0, 35°C, phytase LP2 Lupinus albus
0.634
-
 D-glucose 6-phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
0.634
-
 2-naphthyl phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
0.653
-
 2-naphthyl phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
0.667
-
 2-glycerophosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
0.676
-
 D-fructose 6-phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
0.691
-
 diphosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
0.704
-
 2-glycerophosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
0.718
-
 1-naphthyl phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
0.731
-
 D-fructose 1,6-diphosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
0.74
-
 O-phospho-L-serine pH 5.0, 35°C, phytase LP12 Lupinus albus
0.776
-
 pyridoxal phosphate pH 5.0, 35°C, phytase LP11 Lupinus albus
0.8 11 O-phospho-L-serine pH 5.0, 35°C, phytase LP2 Lupinus albus
0.812
-
 diphosphate pH 5.0, 35°C, phytase LP2 Lupinus albus
0.876
-
 pyridoxal phosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
0.881
-
 2-glycerophosphate pH 5.0, 35°C, phytase LP12 Lupinus albus
0.891
-
 O-phospho-L-serine pH 5.0, 35°C, phytase LP11 Lupinus albus
0.915
-
 pyridoxal phosphate pH 5.0, 35°C, phytase LP2 Lupinus albus

Metals/Ions

Metals/Ions Comment Organism Structure
additional information phytase LP11, LP12 and LP2 are not metalloenzymes Lupinus albus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
 phytase LP11 and LP12, gel filtration Lupinus albus
57000
-
 1 * 57000, phytase LP11 and LP12, SDS-PAGE Lupinus albus
64000
-
 1 * 64000, phytase LP2, SDS-PAGE Lupinus albus
65000
-
 phytase LP2, gel filtration Lupinus albus

Organism

Organism UniProt Comment Textmining
Lupinus albus
-
 var. amiga. The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified.
-

Purification (Commentary)

Purification (Comment) Organism
three phytases LP11, LP12 and LP2 Lupinus albus

Source Tissue

Source Tissue Comment Organism Textmining
seedling 4-day-old Lupinus albus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
498
-
 phytase LP2 Lupinus albus
539
-
 phytase LP11 Lupinus albus
607
-
 phytase LP12 Lupinus albus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O
-
 Lupinus albus 1-naphthol + phosphate
-
 ?
2-glycerophosphate + H2O
-
 Lupinus albus glycerate + phosphate
-
 ?
2-naphthyl phosphate + H2O
-
 Lupinus albus 2-naphthol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Lupinus albus 4-nitrophenol + phosphate
-
 ?
ADP + H2O
-
 Lupinus albus ?
-
 ?
AMP + H2O
-
 Lupinus albus adenosine + phosphate
-
 ?
ATP + H2O
-
 Lupinus albus ?
-
 ?
D-fructose 1,6-diphosphate + H2O
-
 Lupinus albus ?
-
 ?
D-fructose 6-phosphate + H2O
-
 Lupinus albus D-fructose + phosphate
-
 ?
D-glucose 6-phosphate + H2O
-
 Lupinus albus D-glucose + phosphate
-
 ?
diphosphate + H2O
-
 Lupinus albus 2 phosphate
-
 ?
GTP + H2O
-
 Lupinus albus ?
-
 ?
myo-inositol hexakisphosphate + H2O hydrolyzes phytate in a stepwise manner Lupinus albus ? + phosphate
-
 ?
O-phospho-L-serine + H2O
-
 Lupinus albus L-serine + phosphate
-
 ?
pyridoxal phosphate + H2O
-
 Lupinus albus pyridoxal + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 57000, phytase LP11 and LP12, SDS-PAGE Lupinus albus
monomer 1 * 64000, phytase LP2, SDS-PAGE Lupinus albus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
 phytase LP11, LP12 and LP2 Lupinus albus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 90 min, no significant loss of activity at temperatures up ro, phytase LP11, LP12 and LP2 Lupinus albus
50
-
 90 min, 15% loss of activity of phytase, phytase LP11, LP12 and LP2 Lupinus albus
60
-
 90 min, 90 min, 79% loss of activity of phytase LP22, 92% loss of activity of phytase LP12 and 85% loss of activity of phytase LP2 Lupinus albus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Lupinus albus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 phytase LP11, LP12 and LP2 Lupinus albus

pH Range

pH Minimum pH Maximum Comment Organism
3 8 enzyme is virtually inactive below pH 3.0 and above pH 8.0, phytase LP11, LP12 and LP2 Lupinus albus