Cloned (Comment) | Organism |
---|---|
overexpression in Bacillus subtilis using a PHI105MU331 prophage vector system | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, SDS-PAGE | Bacillus subtilis |
47000 | - |
x * 47000, SDS-PAGE | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | Q8KTX7 | The enzyme may be a 3-phytase (EC 3.1.3.8), or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate or alternatively 1D-myo-inositol 1,2,3,5,6-pentakisphosphate has not been identified. | - |
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus licheniformis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | recombinant enzyme in culture medium of Bacillus subtilis | Bacillus licheniformis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
myo-inositol hexakisphosphate + H2O | - |
Bacillus subtilis | ? + phosphate | - |
? | |
myo-inositol hexakisphosphate + H2O | - |
Bacillus licheniformis | ? + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, SDS-PAGE | Bacillus licheniformis |
? | x * 44000, SDS-PAGE | Bacillus subtilis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
- |
Bacillus subtilis |
65 | - |
- |
Bacillus licheniformis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | 65 | 37°C: about 40% of maximal activity, 65°C: about 40% of maximal activity | Bacillus subtilis |
45 | 70 | 45°C: 45% of maximal activity, 70°C: about 55% of maximal activity | Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
10 min, about 15% loss of activity, in presence of 1 mM Ca2+ | Bacillus licheniformis |
70 | - |
10 min, about 70% loss of activity, with 1 mM Ca2+ | Bacillus subtilis |
75 | - |
10 min, about 35% loss of activity, in presence of 1 mM Ca2+ | Bacillus licheniformis |
75 | - |
10 min, about 65% loss of activity, with 1 mM Ca2+ | Bacillus subtilis |
80 | - |
10 min, about 35% loss of activity, in presence of 1 mM Ca2+ | Bacillus licheniformis |
80 | - |
10 min, about 60% loss of activity, with 1 mM Ca2+ | Bacillus subtilis |
85 | - |
10 min, about 30% loss of activity, in presence of 1 mM Ca2+ | Bacillus licheniformis |
85 | - |
10 min, about 45% loss of activity, with 1 mM Ca2+ | Bacillus subtilis |
90 | - |
10 min, about 30% loss of activity, in presence of 1 mM Ca2+ | Bacillus licheniformis |
90 | - |
10 min, about 65% loss of activity, with 1 mM Ca2+ | Bacillus subtilis |
95 | - |
about 40% loss of activity after 15 min, about 60% loss of activity after 30 min, in presence of 5 mM ca2+ | Bacillus licheniformis |
95 | - |
about 55% loss of activity after 15 min, about 75% loss of activity after 30 min, with 5 mM Ca2+ | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
broad | Bacillus subtilis |
6 | - |
broad | Bacillus licheniformis |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Bacillus subtilis | two-dimensional electrophoresis | - |
5 |
Bacillus licheniformis | two-dimensional electrophoresis | - |
5.1 |