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Literature summary for 3.1.3.25 extracted from

  • Ruszkowski, M.; Dauter, Z.
    Structural studies of Medicago truncatula histidinol phosphate phosphatase from inositol monophosphatase superfamily reveal details of penultimate step of histidine biosynthesis in plants (2016), J. Biol. Chem., 291, 9960-9973 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.25

Crystallization (Commentary)

Crystallization (Comment) Organism
structures of complexes with L-histidinol 1-phosphate, L-histidinol, and phosphate as well as the structure showing the cross-linking between two enzyme molecules. The enzymatic reaction requires Mg2+ cations and is catalyzed mainly by amino acid residues from the N-terminal domain. The C-terminal domain is responsible for the substrate specificity Medicago truncatula

Inhibitors

Inhibitors Comment Organism Structure
CO2 enzyme dimers in the presence of CO2 or formaldehyde form mutual, methylene-bridged cross-links between Lys158 and Cys245 residues Medicago truncatula
formaldehyde enzyme dimers in the presence of CO2 or formaldehyde form mutual, methylene-bridged cross-links between Lys158 and Cys245 residues Medicago truncatula
Li+
-
 Medicago truncatula

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.263
-
 L-histidinol phosphate pH 8.4, 23°C Medicago truncatula

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required, optimum concentration 5 mM Medicago truncatula

Organism

Organism UniProt Comment Textmining
Medicago truncatula G7J7Q5
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
proteolytic modification sequence contains a N-terminal signal peptide. The cleavage is predicted to occur between Arg51 and Ala52 Medicago truncatula

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-histidinol phosphate + H2O
-
 Medicago truncatula L-histidinol + phosphate
-
 ?
additional information no substrate: D-myo-inositol-1-phosphate Medicago truncatula ?
-
 ?

Synonyms

Synonyms Comment Organism
11419457
-
 Medicago truncatula

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6
-
 L-histidinol phosphate pH 8.4, 23°C Medicago truncatula

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.4
-
-
 Medicago truncatula

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
5.5
-
 pH 8.4, 23°C Medicago truncatula Li+

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
13.7
-
 L-histidinol phosphate pH 8.4, 23°C Medicago truncatula