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Literature summary for 3.1.3.25 extracted from
- Structural elucidation of the binding site and mode of inhibition of Li+ and Mg2+ in inositol monophosphatase (2014), FEBS J., 281, 5309-5324.
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| hanging drop vapor diffusion method, using 0.2 M MgCl2, 0.1 M Na/HEPES pH 8.0, 18% (w/v) poly(ethyleneglycol) 3350 | Staphylococcus aureus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Li+ | competitive inhibitor of the cofactor Mg2+ | Staphylococcus aureus |  |
| Mg2+ | uncompetitive inhibitor at high concentrations | Staphylococcus aureus | |
| myo-inositol | non-competitive inhibition | Staphylococcus aureus | |
| PO43- | competitive inhibition | Staphylococcus aureus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | dependent on, contains three Mg2+ ions in the active site | Staphylococcus aureus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol phosphate + H2O | Staphylococcus aureus | - |
myo-inositol + phosphate | - |
? | |
| myo-inositol phosphate + H2O | Staphylococcus aureus MSSA476 | - |
myo-inositol + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Staphylococcus aureus | A0A1I9GET0 | - |
- |
| Staphylococcus aureus MSSA476 | A0A1I9GET0 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA affinity column chromatography | Staphylococcus aureus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol phosphate + H2O | - |
Staphylococcus aureus | myo-inositol + phosphate | - |
? | |
| myo-inositol phosphate + H2O | - |
Staphylococcus aureus MSSA476 | myo-inositol + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| IMPase I | - |
Staphylococcus aureus |
| inositol monophosphatase | - |
Staphylococcus aureus |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 81 | - |
Li+ | pH and temperature not specified in the publication | Staphylococcus aureus | |
| 88 | - |
Mg2+ | pH and temperature not specified in the publication | Staphylococcus aureus | |
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Release 2023.1 (January 2023)
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