Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.1.3.25 extracted from

  • Stieglitz, K.A.; Johnson, K.A.; Yang, H.; Roberts, M.F.; Seaton, B.A.; Head, J.F.; Stec, B.
    Crystal structure of a dual activity IMPase/FBPase (AF2372) from Archaeoglobus fulgidus. The story of a mobile loop (2002), J. Biol. Chem., 277, 22863-22874.
    View publication on PubMed
Search for more literature for 3.1.3.25

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Archaeoglobus fulgidus

Crystallization (Commentary)

Crystallization (Comment) Organism
crystallized by vapor diffusion using hanging drops of 0.005 ml. The apoenzyme is crystallized in either 0.2 M ammonium nitrate and 30% PEG 3350 or 0.2 M dihydrogen ammonium phosphate and 30% PEG 3350 Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+
-
 Archaeoglobus fulgidus
Li+
-
 Archaeoglobus fulgidus
additional information 300 mM Na+ or K+have no effect Archaeoglobus fulgidus

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O30298
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
myo-inositol phosphate + H2O
-
 Archaeoglobus fulgidus myo-inositol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
AF2372
-
 Archaeoglobus fulgidus
IMPase/FBPase
-
 Archaeoglobus fulgidus
SuhB
-
 Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
87
-
 Tm-value Archaeoglobus fulgidus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
290
-
 pH 8.0, 85°C Archaeoglobus fulgidus Li+