Literature summary for 3.1.3.25 extracted from

Bhattacharyya, S.; Dutta, D.; Ghosh, A.K.; Das, A.K.
Cloning, overexpression, purification, crystallization and preliminary X-ray diffraction analysis of an inositol monophosphatase family protein (SAS2203) from Staphylococcus aureus MSSA476 (2011), Acta Crystallogr. Sect. F, 67, 471-474.

Search for more literature for 3.1.3.25

Cloned(Commentary)

Cloned (Comment)	Organism
gene sas2203, DNA and amino acid sequence determination and analysis, recombinant expression of the His-tagged enzyme in Escherichia coli strain M15	Staphylococcus aureus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme, sitting drop vapour diffusion method, 24 mg/ml protein in 10 mM Tris-HCl pH 8.0, 50 mM NaCl, 2 mM MgCl2, 5mM DTT is mixed with crystallization solution containing 0.2 M Li2SO4, 20% PEG 3350, 0.1 M HEPES, pH 7.0, method optimization, X-ray diffraction structure determination and analysis at 2.6 A resolution	Staphylococcus aureus

Organism

Organism	UniProt	Comment	Textmining
Staphylococcus aureus	A0A1I9GET0	gene sas2203	-
Staphylococcus aureus MSSA476	A0A1I9GET0	gene sas2203	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged enzyme to homogeneity from Escherichia coli strain M15 by nickel affinity chromatography and gel filtration	Staphylococcus aureus

Synonyms

Synonyms	Comment	Organism
IMPase	-	Staphylococcus aureus
inositol monophosphatase	-	Staphylococcus aureus
More	the enzyme belongs to the FIG superfamily of proteins	Staphylococcus aureus
SAS2203	-	Staphylococcus aureus

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Release 2023.1 (January 2023)