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Literature summary for 3.1.3.25 extracted from

  • Patra, B.; Ghosh Dastidar, K.; Maitra, S.; Bhattacharyya, J.; Majumder, A.L.
    Functional identification of sll1383 from Synechocystis sp PCC 6803 as L-myo-inositol 1-phosphate phosphatase (EC 3.1.3.25): molecular cloning, expression and characterization (2007), Planta, 225, 1547-1558.
    View publication on PubMed
Search for more literature for 3.1.3.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL-21 (DE3) cells Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
Li+
-
 Synechocystis sp.
additional information Na+, K+, NH4+ have no significant effect on the enzyme activity Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0034
-
 DL-myo-inositol 1-phosphate in the presence of 3 mM MgCl2 in 50 mM Tris-Cl (pH 8), at 42°C Synechocystis sp.

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ optimal activation effect at 3 mM Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 SDS-PAGE, recombinant enzyme Synechocystis sp.

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
 strain PCC 6803
-

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose fast flow column chromatography Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-myo-inositol 1-phosphate + H2O
-
 Synechocystis sp. DL-myo-inositol + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
IMPase
-
 Synechocystis sp.
L-myo-inositol 1-phosphate phosphatase
-
 Synechocystis sp.
myo-inositol 1 (or 4) monophosphatase
-
 Synechocystis sp.
sll1383
-
 Synechocystis sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
42
-
 optimally active at about 42°C Synechocystis sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
45 50 more than 30% and 50% of the maximal activity is reduced at 45°C and 50°C, respectively Synechocystis sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 at 37°C IMPase activity of sll1383 shows about 45% of the activity observed at 42°C Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 in 50 mM Tris-Cl buffer Synechocystis sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9.5 about 50% of the maximum activity is retained at pH 6.0 and pH 9.5 Synechocystis sp.

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6
-
 in the presence of 3 mM MgCl2 in 50 mM Tris-Cl (pH 8), at 42°C Synechocystis sp. Li+