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Literature summary for 3.1.3.25 extracted from

  • Fukuda, C.; Kawai, S.; Murata, K.
    NADP(H) phosphatase activities of archaeal inositol monophosphatase and eubacterial 3-phosphoadenosine 5-phosphate phosphatase (2007), Appl. Environ. Microbiol., 73, 5447-5452.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.25

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Methanocaldococcus jannaschii
expressed in Escherichia coli Origami B(DE3) cells Archaeoglobus fulgidus
expressed in Escherichia coli strain K-12 AG1 Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
LiCl
-
 Archaeoglobus fulgidus
LiCl
-
 Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.18
-
 D-myo-Inositol 1-phosphate
-
 Archaeoglobus fulgidus
1.02
-
 D-myo-Inositol 1-phosphate
-
 Methanocaldococcus jannaschii

Metals/Ions

Metals/Ions Comment Organism Structure
Li+ 127% activity in the presence of 500 mM Li+ Methanocaldococcus jannaschii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
28000
-
 SDS-PAGE Archaeoglobus fulgidus
28600
-
 SDS-PAGE Methanocaldococcus jannaschii

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus O30298
-
-
Escherichia coli
-
-
-
Methanocaldococcus jannaschii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Toyopearl Super Q-650S column chromatography, phenyl-Sepharose column chromatography, ultracentrifugation, and Sephacryl S-200 gel filtration Methanocaldococcus jannaschii
Toyopearl Super Q-650S column chromatography, ultracentrifugation, and Sephacryl S-200 gel filtration Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2'-AMP + H2O
-
 Methanocaldococcus jannaschii adenosine + phosphate
-
 ?
2'-AMP + H2O
-
 Archaeoglobus fulgidus adenosine + phosphate
-
 ?
2'-phosphoadenosine 5'-phosphate + H2O
-
 Methanocaldococcus jannaschii ?
-
 ?
2'-phosphoadenosine 5'-phosphate + H2O
-
 Archaeoglobus fulgidus ?
-
 ?
D-fructose 1-phosphate + H2O
-
 Methanocaldococcus jannaschii D-fructose + phosphate
-
 ?
D-fructose 1-phosphate + H2O
-
 Archaeoglobus fulgidus D-fructose + phosphate
-
 ?
D-glucose 1-phosphate + H2O
-
 Methanocaldococcus jannaschii D-glucose + phosphate
-
 ?
D-glucose 1-phosphate + H2O
-
 Archaeoglobus fulgidus D-glucose + phosphate
-
 ?
D-myo-inositol 1,4-bisphosphate + H2O
-
 Methanocaldococcus jannaschii ?
-
 ?
D-myo-inositol 1,4-bisphosphate + H2O
-
 Archaeoglobus fulgidus ?
-
 ?
D-myo-inositol 1-phosphate + H2O
-
 Escherichia coli D-myo-inositol + phosphate
-
 ?
D-myo-inositol 1-phosphate + H2O
-
 Methanocaldococcus jannaschii D-myo-inositol + phosphate
-
 ?
D-myo-inositol 1-phosphate + H2O
-
 Archaeoglobus fulgidus D-myo-inositol + phosphate
-
 ?
D-myo-inositol 2-phosphate + H2O
-
 Methanocaldococcus jannaschii D-myo-inositol + phosphate
-
 ?
D-myo-inositol 2-phosphate + H2O
-
 Archaeoglobus fulgidus D-myo-inositol + phosphate
-
 ?
additional information D-myo-inositol-1,4,5-triphosphate, D-glucose 6-phosphate, D-fructose 6-phosphate, NAD+, FMN+, bis-p-nitrophenyl diphosphate, ADP and ATP are no substrates Methanocaldococcus jannaschii ?
-
 ?
additional information D-myo-inositol-1,4,5-triphosphate, D-glucose 6-phosphate, D-fructose 6-phosphate, NAD+, FMN+, bis-p-nitrophenyl diphosphate, ADP and ATP are no substrates Archaeoglobus fulgidus ?
-
 ?
additional information the enzyme also exhibits high phosphatase activities toward NADP(H) and toward 2'-AMP and 2'-phosphoadenosine 5'-phosphate, but not toward 3'-AMP, 3'-phosphoadenosine 5'-phosphate, and 5'-AMP Methanocaldococcus jannaschii ?
-
 ?
additional information the enzyme also exhibits high phosphatase activities toward NADP(H) and toward 2'-AMP and 2'-phosphoadenosine 5'-phosphate, but not toward 3'-AMP, 3'-phosphoadenosine 5'-phosphate, and 5'-AMP Archaeoglobus fulgidus ?
-
 ?
p-nitrophenyl phosphate + H2O
-
 Methanocaldococcus jannaschii p-nitrophenol + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Archaeoglobus fulgidus p-nitrophenol + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer
-
 Methanocaldococcus jannaschii
dimer
-
 Archaeoglobus fulgidus

Synonyms

Synonyms Comment Organism
AF2372 exhibits NADP(H) phosphatase and fructose-1,6-bisphosphatase activities in addition to inositol monophosphatase activitity, but not 3'-phosphoadenosine 5'-phosphate phosphatase activity Archaeoglobus fulgidus
IMPase
-
 Escherichia coli
IMPase
-
 Methanocaldococcus jannaschii
IMPase
-
 Archaeoglobus fulgidus
inositol phosphatase
-
 Escherichia coli
inositol phosphatase
-
 Methanocaldococcus jannaschii
inositol phosphatase
-
 Archaeoglobus fulgidus
MJ0109 exhibits NADP(H) phosphatase and fructose-1,6-bisphosphatase activities in addition to the inositol monophosphatase activity, but not 3'-phosphoadenosine 5'-phosphate phosphatase activity Methanocaldococcus jannaschii
SuhB
-
 Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1
-
 D-myo-Inositol 1-phosphate
-
 Methanocaldococcus jannaschii
28.7
-
 D-myo-Inositol 1-phosphate
-
 Archaeoglobus fulgidus

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
30
-
-
 Escherichia coli LiCl
30
-
-
 Archaeoglobus fulgidus LiCl