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Literature summary for 3.1.3.25 extracted from
- Overexpression, purification, and analysis of complementation behavior of E. coli SuhB protein: Comparison with bacterial and archaeal inositol monophosphatases (2000), Biochemistry, 39, 4145-4153.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Eschericha coli inositol monophosphatase deficient strain CG1307, can not complement effects of inositol monophosphatase deficiency | Methanocaldococcus jannaschii |
| expressed in Eschericha coli inositol monophosphatase deficient strain CG1307, can not complement effects of inositol monophosphatase deficiency | Archaeoglobus fulgidus |
| expressed in Eschericha coli inositol monophosphatase deficient strain CG1307, can not complement effects of inositol monophosphatase deficiency | Thermotoga maritima |
| expressed in Eschericha coli inositol monophosphatase deficient strain CG1307, complements effects of inositol monophosphatase deficiency, expressed in Escherichia coli BL21-(DE3)pLsyS | Escherichia coli |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D87N | complete inactivation, resulting enzyme is still able to complement inositol monophosphatase deficiency in Escherichia coli CG1307 | Escherichia coli |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Li+ | - |
Escherichia coli |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.064 | - |
myo-inositol 1-phosphate | pH 8.0, 37°C | Escherichia coli | |
| 0.079 | - |
L-inositol 1-phosphate | pH 8.0, 37°C | Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | partial activation | Escherichia coli | |
| Fe2+ | very weak activation | Escherichia coli | |
| Li+ | very potent inhibitor | Escherichia coli | |
| Mg2+ | required, highest activity with 6 mM Mg2+ | Escherichia coli | |
| Mn2+ | partial activation | Escherichia coli | |
| Ni2+ | partial activation | Escherichia coli | |
| Zn2+ | very weak activation | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29200 | - |
calculated from amino acid sequence | Escherichia coli |
| 30500 | - |
gel filtration at low ionic strength | Escherichia coli |
| 32000 | - |
SDS-PAGE | Escherichia coli |
| 39000 | - |
gel filtration in the presence of 250 mM KCl | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| myo-inositol 1-phosphate + H2O | Methanocaldococcus jannaschii | - |
myo-inositol + phosphate | - |
? | |
| myo-inositol 1-phosphate + H2O | Archaeoglobus fulgidus | - |
myo-inositol + phosphate | - |
? | |
| myo-inositol 1-phosphate + H2O | Thermotoga maritima | - |
myo-inositol + phosphate | - |
? | |
| myo-inositol 1-phosphate + H2O | Escherichia coli | very low concentration of inositol compounds | myo-inositol + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Archaeoglobus fulgidus | - |
- |
- |
| Escherichia coli | - |
- |
- |
| Methanocaldococcus jannaschii | - |
- |
- |
| Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| high yield purification of recombinant protein from Escherichia coli BL21-(DE3)pLsyS | Escherichia coli |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.3 | - |
purified enzyme, pH 8.0, 37°C | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2'-AMP + H2O | - |
Escherichia coli | adenosine + phosphate | - |
? | |
| glycerol 2-phosphate + H2O | - |
Escherichia coli | glycerol + phosphate | - |
? | |
| additional information | no activity with 4-nitrophenyl phosphate, 5'-AMP, NAD+, glucose 1-phosphate and glucose 6-phosphate | Escherichia coli | ? | - |
? | |
| myo-inositol 1-phosphate + H2O | - |
Methanocaldococcus jannaschii | myo-inositol + phosphate | - |
? | |
| myo-inositol 1-phosphate + H2O | - |
Archaeoglobus fulgidus | myo-inositol + phosphate | - |
? | |
| myo-inositol 1-phosphate + H2O | - |
Thermotoga maritima | myo-inositol + phosphate | - |
? | |
| myo-inositol 1-phosphate + H2O | D- and L-isomer, 2.5-fold higher activity with D-isomer | Escherichia coli | myo-inositol + phosphate | - |
? | |
| myo-inositol 1-phosphate + H2O | very low concentration of inositol compounds | Escherichia coli | myo-inositol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | SDS-PAGE and gel filtration | Escherichia coli |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Escherichia coli |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | 100 | - |
Escherichia coli |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
10% of activity lost within 5 min | Escherichia coli |
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