Cloned (Comment) | Organism |
---|---|
expression of the MJ109 gene in Escherichia coli | Methanocaldococcus jannaschii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Methanocaldococcus jannaschii | |
Ca2+ | - |
Methanocaldococcus jannaschii | |
Co2+ | - |
Methanocaldococcus jannaschii | |
Cu2+ | - |
Methanocaldococcus jannaschii | |
Li+ | 250 mM inhibits about 65%, 1000 mM about 90% | Methanocaldococcus jannaschii | |
Ni2+ | - |
Methanocaldococcus jannaschii | |
Zn2+ | - |
Methanocaldococcus jannaschii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Li+ | at 100 mM the activity is slightly enhanced | Methanocaldococcus jannaschii | |
Mg2+ | - |
Methanocaldococcus jannaschii | |
Mn2+ | - |
Methanocaldococcus jannaschii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
2 * 28000, SDS-PAGE | Methanocaldococcus jannaschii |
55000 | - |
gel filtration | Methanocaldococcus jannaschii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methanocaldococcus jannaschii | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.3 | - |
- |
Methanocaldococcus jannaschii |
Storage Stability | Organism |
---|---|
4°C, no loss of activity for 1 month | Methanocaldococcus jannaschii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-AMP + H2O | - |
Methanocaldococcus jannaschii | adenosine + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | high rate of hydrolysis | Methanocaldococcus jannaschii | 4-nitrophenol + phosphate | - |
? | |
glucose 1-phosphate + H2O | high rate of hydrolysis | Methanocaldococcus jannaschii | ? | - |
? | |
glycerol 2-phosphate + H2O | - |
Methanocaldococcus jannaschii | glycerol + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 28000, SDS-PAGE | Methanocaldococcus jannaschii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
for 30 min 95% of activity remains | Methanocaldococcus jannaschii |