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Literature summary for 3.1.3.24 extracted from

  • Lunn, J.E.; Ashton, A.R.; Hatch, M.D.; Heldt, H.W.
    Purification, molecular cloning, and sequence analysis of sucrose-6F-phosphate phosphohydrolase from plants (2000), Proc. Natl. Acad. Sci. USA, 97, 12914-12919.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.24

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Zea mays

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
 Oryza sativa
sucrose weak competitive inhibition Oryza sativa
Zn2+
-
 Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.065
-
 sucrose 6-phosphate pH 7.0, 30°C Oryza sativa

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ highly dependent on, can partially be replaced by Mn2+ Oryza sativa
Mn2+ can partially replace Mg2+, 37% of the activity with Mg2+ at 0.1 mM Oryza sativa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47213
-
 x * 47213, DNA sequence calculation Zea mays
50000
-
 2 * 50000, SDS-PAGE Oryza sativa
100000
-
 gel filtration Oryza sativa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sucrose 6-phosphate + H2O Oryza sativa final step of sucrose biosynthesis sucrose + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-
Zea mays Q9FQ11
-
-

Purification (Commentary)

Purification (Comment) Organism
25000fold, to homogeneity Oryza sativa

Reaction

Reaction Comment Organism Reaction ID
sucrose 6F-phosphate + H2O = sucrose + phosphate N-terminal catalytic domain Zea mays
a

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Oryza sativa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.37
-
 recombinant enzyme in extract of expressing Escherichia coli Zea mays
1250
-
 purified enzyme Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with fructose 6-phosphate Oryza sativa ?
-
 ?
sucrose 6-phosphate + H2O
-
 Zea mays sucrose + phosphate
-
 ?
sucrose 6-phosphate + H2O highly specific for Oryza sativa sucrose + phosphate
-
 ?
sucrose 6-phosphate + H2O final step of sucrose biosynthesis Oryza sativa sucrose + phosphate
-
 ?

Subunits

Subunits Comment Organism
? x * 47213, DNA sequence calculation Zea mays
dimer 2 * 50000, SDS-PAGE Oryza sativa

Synonyms

Synonyms Comment Organism
SPP
-
 Oryza sativa
SPP
-
 Zea mays