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Literature summary for 3.1.3.23 extracted from

  • Guggisberg, A.M.; Park, J.; Edwards, R.L.; Kelly, M.L.; Hodge, D.M.; Tolia, N.H.; Odom, A.R.
    A sugar phosphatase regulates the methylerythritol phosphate (MEP) pathway in malaria parasites (2014), Nat. Commun., 5, 4467.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.23

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3)pLysS cells Plasmodium falciparum

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.1M HEPES (pH 7.5) and 20-25% (w/v) PEG 8000 Plasmodium falciparum

Protein Variants

Protein Variants Comment Organism
N70S the mutant shows wild type activity Plasmodium falciparum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Plasmodium falciparum 5737
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ contains Mg2+ in the active site Plasmodium falciparum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
 x * 33000, SDS-PAGE Plasmodium falciparum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose 1-phosphate + H2O Plasmodium falciparum best substrate D-fructose + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Plasmodium falciparum Q8IJ74
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel agarose bead chromatography and Superdex 200 gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Plasmodium falciparum 4-nitrophenol + phosphate
-
 ?
D-erythrose 4-phosphate + H2O
-
 Plasmodium falciparum D-erythrose + phosphate
-
 ?
D-fructose 1-phosphate + H2O best substrate Plasmodium falciparum D-fructose + phosphate
-
 ?
D-fructose 6-phosphate + H2O
-
 Plasmodium falciparum D-fructose + phosphate
-
 ?
D-glucose 1-phosphate + H2O
-
 Plasmodium falciparum D-glucose + phosphate
-
 ?
D-glucose 2-phosphate + H2O second best substrate Plasmodium falciparum D-glucose + phosphate
-
 ?
D-glucose 6-phosphate + H2O
-
 Plasmodium falciparum D-glucose + phosphate
-
 ?
D-mannose 6-phosphate + H2O
-
 Plasmodium falciparum D-mannose + phosphate
-
 ?
D-ribose 5-phosphate + H2O
-
 Plasmodium falciparum D-ribose + phosphate
-
 ?
D-ribulose 5-phosphate + H2O
-
 Plasmodium falciparum D-ribulose + phosphate
-
 ?
deoxyribose 5-phosphate + H2O
-
 Plasmodium falciparum deoxyribose + phosphate
-
 ?
deoxyxylulose 5-phosphate + H2O
-
 Plasmodium falciparum deoxyxylulose + phosphate
-
 ?
dihydroxyacetone phosphate + H2O
-
 Plasmodium falciparum dihydroxyacetone + phosphate
-
 ?
methylerythritol phosphate + H2O
-
 Plasmodium falciparum methylerythritol + phosphate
-
 ?
additional information low activity towards D-fructose 1,6-bisphosphate, D-galactose 1-phosphate, glycerol 2-phosphate, glucosamine 1-phosphate, D-mannose 1-phosphate, phosphoenolpyruvate, sedoheptulose 7-phosphate, sorbitol 6-phosphate, trehalose 6-phosphate, 2-phosphoglyceric acid, and 3-phosphoglyceric acid Plasmodium falciparum ?
-
 ?
additional information the enzyme is most active against monophosphorylated three to six-carbon monosaccharides Plasmodium falciparum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 33000, SDS-PAGE Plasmodium falciparum

Synonyms

Synonyms Comment Organism
HAD1
-
 Plasmodium falciparum
haloacid dehalogenase-like sugar phosphatase
-
 Plasmodium falciparum
PF3D7 1033400 locus name Plasmodium falciparum
sugar phosphatase
-
 Plasmodium falciparum

General Information

General Information Comment Organism
malfunction loss of HAD1 is required for fosmidomycin resistance. Parasites lacking HAD1 have increased methylerythritol phosphate pathway metabolites, particularly the deoxyxylulose 5-phosphate reductoisomerase substrate deoxyxylulose 5-phosphate Plasmodium falciparum
metabolism HAD1 controls substrate availability to the methylerythritol phosphate pathway Plasmodium falciparum