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Literature summary for 3.1.3.21 extracted from
- Glycerol synthesis in freeze-resistant rainbow smelt: towards the characterization of a key enzyme glycerol-3-phosphatase (2014), Fish Physiol. Biochem., 40, 257-266.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Osmerus mordax |  |
| dithiothreitol | - |
Osmerus mordax | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.61 | - |
glycerol 3-phosphate | at pH 5.5, temperature not specified in the publication | Osmerus mordax | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytosol | - |
Osmerus mordax | 5829 | - |
| lysosome | - |
Osmerus mordax | 5764 | - |
| peroxisome | - |
Osmerus mordax | 5777 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | strong requirement for Mg2+, 5 mM used in assay conditions. Mg2+ induces a 5-6fold increaseof activity for concentrations higher than 1 mM | Osmerus mordax | |
| Mn2+ | Mn2+ induces a 2-2.5fold increase of activity for concentrations higher than 0.5 mM | Osmerus mordax | |
| Zn2+ | activity is increased by 6fold in the presence of 0.5 mM Zn2+ | Osmerus mordax | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol 3-phosphate + H2O | Osmerus mordax | - |
glycerol + phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Osmerus mordax | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| UNOsphere Q column chromatography, anion-exchange column chromatography, and Superdex 200 gel filtration | Osmerus mordax |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Osmerus mordax | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glycerol 3-phosphate + H2O | - |
Osmerus mordax | glycerol + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| G3Pase | - |
Osmerus mordax |
| glycerol-3-phosphatase | - |
Osmerus mordax |
| phosphatase, glycerol-3-phosphatase | - |
Osmerus mordax |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Osmerus mordax |
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Release 2023.1 (January 2023)
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