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Literature summary for 3.1.3.2 extracted from
- Purification and characterization of a novel acid phosphatase from the split gill mushroom Schizophyllum commune (2013), J. Basic Microbiol., 53, 868-875.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | 0.5 mM, 19% residual activity | Schizophyllum commune |  |
| F- | 0.5 mM, 45% residual activity | Schizophyllum commune | |
| Fe3+ | 0.5 mM, 42.5% residual activity | Schizophyllum commune | |
| additional information | not inhibitory: EDTA | Schizophyllum commune |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.248 | - |
4-nitrophenyl phosphate | pH 4.6, 37°C | Schizophyllum commune | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 0.5 mM, 116% of initial activiy | Schizophyllum commune | |
| Co2+ | 0.5 mM, 125% of initial activiy | Schizophyllum commune | |
| Mg2+ | 0.5 mM, 117% of initial activiy | Schizophyllum commune | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 72500 | - |
gel filtration | Schizophyllum commune |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizophyllum commune | B3EWC6 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Schizophyllum commune |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate | - |
Schizophyllum commune | 4-nitrophenol + phosphate | - |
? | |
| additional information | no activity towards substrates sodium phytate, ATP, ADP, AMP, glucose 6-phosphate, fructose 6-phosphate, and beta-glycerophosphate | Schizophyllum commune | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 72500, SDS-PAGE | Schizophyllum commune |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Schizophyllum commune |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 40 | - |
Schizophyllum commune |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
- |
Schizophyllum commune |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | 5.6 | - |
Schizophyllum commune |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 8 | - |
Schizophyllum commune |
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