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Literature summary for 3.1.3.2 extracted from

  • Tran, H.T.; Qian, W.; Hurley, B.A.; She, Y.M.; Wang, D.; Plaxton, W.C.
    Biochemical and molecular characterization of AtPAP12 and AtPAP26: the predominant purple acid phosphatase isozymes secreted by phosphate-starved Arabidopsis thaliana (2010), Plant Cell Environ., 33, 1789-1803.
    View publication on PubMed
Search for more literature for 3.1.3.2

Inhibitors

Inhibitors Comment Organism Structure
fluoride inhibits activity Arabidopsis thaliana
molybdate
-
 Arabidopsis thaliana
vanadate
-
 Arabidopsis thaliana
Zn2+ inhibits activity Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.059
-
 phosphoenolpyruvate pH 5.6, 25°C Arabidopsis thaliana
0.178
-
 phosphoenolpyruvate pH 5.6, 25°C Arabidopsis thaliana

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ enhances activity Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 SDS-PAGE Arabidopsis thaliana
60000
-
 SDS-PAGE Arabidopsis thaliana
130000
-
 gel filtration, native molecular mass Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Arabidopsis thaliana
glycoprotein differential glycosylation influences the subcellular targeting and substrate selectivity of AtPAP26 Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
-
 Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O relative activity: 43% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana 1-naphthol + phosphate
-
 ?
1-naphthyl phosphate + H2O relative activity: 53% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana 1-naphthol + phosphate
-
 ?
2-naphthyl phosphate + H2O relative activity: 116% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana 2-naphthol + phosphate
-
 ?
2-naphthyl phosphate + H2O relative activity: 118% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana 2-naphthol + phosphate
-
 ?
3-phosphoglycerate + H2O relative activity: 38% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana glycerate + phosphate
-
 ?
3-phosphoglycerate + H2O relative activity: 67% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana glycerate + phosphate
-
 ?
4-nitrophenyl phosphate + H2O relative activity: 101% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O relative activity: 83% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana 4-nitrophenol + phosphate
-
 ?
6-phospho-D-gluconate + H2O relative activity: 67% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana gluconate + phosphate
-
 ?
6-phospho-D-gluconate + H2O relative activity: 69% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana gluconate + phosphate
-
 ?
ADP + H2O relative activity: 68% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana AMP + phosphate
-
 ?
ADP + H2O relative activity: 77% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana AMP + phosphate
-
 ?
ATP + H2O relative activity: 52% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana ADP + phosphate
-
 ?
ATP + H2O relative activity: 81% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana ADP + phosphate
-
 ?
D-ribose 3-phosphate + H2O relative activity: 25% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana D-ribose + phosphate
-
 ?
D-ribose 3-phosphate + H2O relative activity: 41% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana D-ribose + phosphate
-
 ?
D-ribose 5-phosphate + H2O relative activity: 50% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana D-ribose + phosphate
-
 ?
D-ribose 5-phosphate + H2O relative activity: 9% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana D-ribose + phosphate
-
 ?
dAMP + H2O relative activity: 12% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana deoxyadenosine + phosphate
-
 ?
dAMP + H2O relative activity: 19% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana deoxyadenosine + phosphate
-
 ?
glycerol 3-phosphate + H2O relative activity: 44% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana glycerol + phosphate
-
 ?
glycerol 3-phosphate + H2O relative activity: 59% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana glycerol + phosphate
-
 ?
GTP + H2O relative activity: 29% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana GDP + phosphate
-
 ?
GTP + H2O relative activity: 45% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana GDP + phosphate
-
 ?
L-phosphoserine + H2O relative activity: 14% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana L-serine + phosphate
-
 ?
L-phosphoserine + H2O relative activity: 26% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana L-serine + phosphate
-
 ?
L-phosphothreonine + H2O relative activity: 29% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana L-threonine + phosphate
-
 ?
L-phosphothreonine + H2O relative activity: 49% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana L-threonine + phosphate
-
 ?
Na diphosphate + H2O relative activity: 41% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana ?
-
 ?
Na diphosphate + H2O relative activity: 63% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana ?
-
 ?
phenyl phosphate + H2O relative activity: 96% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana phenol + phosphate
-
 ?
phenyl phosphate + H2O relative activity: 98% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana phenol + phosphate
-
 ?
phosphoenolpyruvate + H2O relative activity: 100% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana pyruvate + phosphate
-
 ?
tyrosine phosphate + H2O relative activity: 60% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana tyrosine + phosphate
-
 ?
tyrosine phosphate + H2O relative activity: 71% compared to phosphoenolpyruvate as substrate Arabidopsis thaliana tyrosine + phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer gel filtration, 2 * 60000 Arabidopsis thaliana
monomer 1 * 55000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
acid phosphatase
-
 Arabidopsis thaliana
AtPAP12
-
 Arabidopsis thaliana
AtPAP26
-
 Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
 Arabidopsis thaliana
5.6
-
 assay at Arabidopsis thaliana

pH Range

pH Minimum pH Maximum Comment Organism
7 8 significant activity Arabidopsis thaliana

Expression

Organism Comment Expression
Arabidopsis thaliana AtPAP26 is markedly up-regulated in response to phosphate deprivation up

General Information

General Information Comment Organism
malfunction analysis of atpap12 and atpap26 T-DNA mutants verifies that AtPAP12 and AtPAP26 account for most of the secreted acid phosphatase activity of phosphate-deficient wild-type seedlings Arabidopsis thaliana
physiological function AtPAP26 is dual targeted during phosphate stress because it is also the principal intracellular (vacuolar) PAP up-regulated by phosphate-deficient Arabidopsis Arabidopsis thaliana