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Literature summary for 3.1.3.2 extracted from
- Identification, characterization and partial purification of acid phosphatase from cotyledons of Psoralea corylifolia L (2011), Am. J. Plant Physiol., 6, 228-241.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | inhibits enzymatic activity | Cullen corylifolium |  |
| NaF | inhibits enzymatic activity | Cullen corylifolium | |
| Sodium molybdate | inhibits enzymatic activity | Cullen corylifolium | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.775 | - |
4-nitrophenyl phosphate | pH 5.5, 37°C | Cullen corylifolium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28000 | - |
two spots detected in two-dimensional gel electrophoresis followed by western blot analysis | Cullen corylifolium |
| 30000 | - |
two spots detected in two-dimensional gel electrophoresis followed by western blot analysis | Cullen corylifolium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cullen corylifolium | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| (NH4)2SO4 precipitation, DEAE cellulose separation and gel filtration | Cullen corylifolium |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Cullen corylifolium | - |
| embryonic axis | - |
Cullen corylifolium | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 264200 | - |
purified enzyme, pH 5.5, 37°C | Cullen corylifolium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl phosphate + H2O | - |
Cullen corylifolium | 4-nitrophenol + phosphate | - |
? | |
| ATP + H2O | - |
Cullen corylifolium | ADP + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | - |
Cullen corylifolium | D-glucose + phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| acid phosphatase | - |
Cullen corylifolium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Cullen corylifolium |
| 50 | - |
optimal temperature | Cullen corylifolium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Cullen corylifolium |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 5.5 | - |
Cullen corylifolium |
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