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Literature summary for 3.1.3.2 extracted from

  • Zhang, G.Q.; Dong, X.F.; Wang, Z.H.; Zhang, Q.; Wang, H.X.; Tong, J.M.
    Purification, characterization, and cloning of a novel phytase with low pH optimum and strong proteolysis resistance from Aspergillus ficuum NTG-23 (2010), Biores. Technol., 101, 4125-4131.
    View publication on PubMed
Search for more literature for 3.1.3.2

Activating Compound

Activating Compound Comment Organism Structure
EDTA slight stimulation Aspergillus ficuum

Cloned(Commentary)

Cloned (Comment) Organism
-
 Aspergillus ficuum

General Stability

General Stability Organism
enzyme shows strong resistance toward pepsin and trypsin Aspergillus ficuum

Metals/Ions

Metals/Ions Comment Organism Structure
additional information no effect: Ca2+, Mg2+, Mn2+, Zn2+ Aspergillus ficuum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65500
-
 gel filtration Aspergillus ficuum

Organism

Organism UniProt Comment Textmining
Aspergillus ficuum
-
-
-
Aspergillus ficuum NTG-23
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
150
-
 37°C, pH 2.5 Aspergillus ficuum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-glycerophosphate + H2O 127% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum glycerate + phosphate
-
 ?
4-nitrophenyl phosphate + H2O 254% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum 4-nitrophenol + phosphate
-
 ?
ADP + H2O 177% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum ? + phosphate
-
 ?
AMP + H2O 173% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum adenosine + phosphate
-
 ?
ATP + H2O 256% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum ? + phosphate
-
 ?
D-fructose 6-phosphate + H2O 228% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum D-fructose + phosphate
-
 ?
D-glucose 6-phosphate + H2O 190% of the activity with myo-inositol hexakisphosphate Aspergillus ficuum D-glucose + phosphate
-
 ?
myo-inositol hexakisphosphate + H2O sodium salt Aspergillus ficuum 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 65500, SDS-PAGE Aspergillus ficuum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
67
-
-
 Aspergillus ficuum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
70
-
 20 min, no loss of activity Aspergillus ficuum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3
-
-
 Aspergillus ficuum