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Literature summary for 3.1.3.2 extracted from
- Molecular and biochemical characterization of AtPAP15, a purple acid phosphatase with phytase activity, in Arabidopsis (2009), Plant Physiol., 151, 199-209.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Nicotiana tabacum | Arabidopsis thaliana |
| His-tagged version expressed inNicotiana tabacum, GST-fusion protein (without any enzyme activity) expressed in Escherichia coli | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 17.8% of initial activity; inhibites phytase activity | Arabidopsis thaliana |  |
| F- | reduces phytase activity | Arabidopsis thaliana | |
| molybdate | 0.25 mM: enzymatic activity completely abolished | Arabidopsis thaliana | |
| additional information | neither inhibitory nor stimulating: Mn2+, Mg2+, Ni2+, SO4-, and NO3- | Arabidopsis thaliana | |
| PO43- | reduces phytase activity | Arabidopsis thaliana | |
| SO3- | 43.1% of initial activity | Arabidopsis thaliana | |
| SO32- | inhibites phytase activity | Arabidopsis thaliana | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.278 | - |
sodium phytate | - |
Arabidopsis thaliana | |
| 0.278 | - |
myo-inositol hexakisphosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
| 0.703 | - |
p-nitrophenyl phosphate | - |
Arabidopsis thaliana | |
| 0.703 | - |
4-nitrophenyl phosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
| 0.801 | - |
phosphoenolpyruvate | - |
Arabidopsis thaliana | |
| 0.801 | - |
phosphoenolpyruvate | 37°C, pH 4.5 | Arabidopsis thaliana | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | stimulates phytase activity | Arabidopsis thaliana | |
| Ca2+ | 127% of initial activity | Arabidopsis thaliana | |
| Zn2+ | stimulates phytase activity | Arabidopsis thaliana | |
| Zn2+ | 129.4%, of initial activity | Arabidopsis thaliana | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 58000 | - |
gel filtration | Arabidopsis thaliana |
| 60000 | - |
His-tagged protein, SDS-PAGE, gel filtration | Arabidopsis thaliana |
| 60000 | - |
1 * 60000, SDS-PAGE | Arabidopsis thaliana |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
purple acid phosphatase with phytase activity | - |
| Arabidopsis thaliana | Q9SFU3 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cation-exchange chromatography, Ni2+ affinity chromatography, gel filtration | Arabidopsis thaliana |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| pollen | strong expression in the early stages of seedling growth and pollen germination | Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
| seedling | strong expression in the early stages of seedling growth and pollen germination | Arabidopsis thaliana | - |
| vasculature | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 10 | - |
37°C, pH 4.5 | Arabidopsis thaliana |
| 13.4 | - |
substrate: sodium phytate | Arabidopsis thaliana |
| 134.7 | - |
substrate: phosphoenolpyruvate | Arabidopsis thaliana |
| 165.4 | - |
substrate: p-nitrophenyl phosphate | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 3-phosphoglycerate + H2O | 69% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | glycerate + phosphate | - |
? | |
| 4-nitrophenyl phosphate + H2O | - |
Arabidopsis thaliana | 4-nitrophenol + phosphate | - |
? | |
| ADP + H2O | 50% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
| D-glucose 6-phosphate + H2O | 20% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | D-glucose + phosphate | - |
? | |
| dATP + H2O | 31% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
| dCTP + H2O | 35% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
| dGTP + H2O | 52% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
| diphosphate + H2O | - |
Arabidopsis thaliana | 2 phosphate | - |
? | |
| diphosphate + H2O | 95% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | 2 phosphate | - |
? | |
| dTTP + H2O | 37% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | ? + phosphate | - |
? | |
| glycerol 3-phosphate + H2O | 43% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | glycerol + phosphate | - |
? | |
| myo-inositol hexakisphosphate + H2O | 7% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + phosphate | - |
? | |
| O-phospho-L-serine + H2O | 25% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | L-serine + phosphate | - |
? | |
| O-phospho-L-tyrosine + H2O | 28% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | L-tyrosine + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | further substrates: 3-phosphoglyceric acid, ADP, dGTP, glucose 3-phosphate, dTTP, dCTP, dATP, O-phospho-Tyr, O-phospho-Ser, glucose 6-phosphate, CMP, O-phospho-Thr, ribose 5-phosphate, ATP, fructose 6-phosphate, AMP, GMP | Arabidopsis thaliana | p-nitrophenol + phosphate | - |
? | |
| phosphoenolpyruvate + H2O | - |
Arabidopsis thaliana | pyruvate + phosphate | - |
? | |
| phosphoenolpyruvate + H2O | 92% of the activity with 4-nitrophenylphosphate | Arabidopsis thaliana | pyruvate + phosphate | - |
? | |
| phytate + H2O | - |
Arabidopsis thaliana | ? + phosphate | - |
? | |
| sodium phytate + H2O | - |
Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 60000, SDS-PAGE | Arabidopsis thaliana |
| monomer | 1 * 60000, His-tagged protein, SDS-PAGE, gel filtration | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| AtPAP15 | - |
Arabidopsis thaliana |
| purple acid phosphatase | - |
Arabidopsis thaliana |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | 37 | - |
Arabidopsis thaliana |
| 23 | 37 | highest activity | Arabidopsis thaliana |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 23 | 37 | most stable | Arabidopsis thaliana |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 8.85 | - |
sodium phytate | - |
Arabidopsis thaliana | |
| 8.85 | - |
myo-inositol hexakisphosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
| 161.9 | - |
p-nitrophenyl phosphate | - |
Arabidopsis thaliana | |
| 161.9 | - |
4-nitrophenyl phosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
| 198 | - |
phosphoenolpyruvate | 37°C, pH 4.5 | Arabidopsis thaliana | |
| 198.6 | - |
phosphoenolpyruvate | - |
Arabidopsis thaliana | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Arabidopsis thaliana |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Arabidopsis thaliana | expressed in the vasculature, pollen grains and roots | additional information |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | gene disruption mutants show a lower phytase and phophatase activity in seedlings and germinating pollen and lower pollen germination rate | Arabidopsis thaliana |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 26.7 | - |
sodium phytate | - |
Arabidopsis thaliana | |
| 31.8 | - |
myo-inositol hexakisphosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
| 232 | - |
4-nitrophenyl phosphate | 37°C, pH 4.5 | Arabidopsis thaliana | |
| 241.3 | - |
p-nitrophenyl phosphate | - |
Arabidopsis thaliana | |
| 248 | - |
phosphoenolpyruvate | 37°C, pH 4.5 | Arabidopsis thaliana | |
| 253.8 | - |
phosphoenolpyruvate | - |
Arabidopsis thaliana | |
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