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Literature summary for 3.1.3.2 extracted from

  • Reilly, T.J.; Chance, D.L.; Calcutt, M.J.; Tanner, J.J.; Felts, R.L.; Waller, S.C.; Henzl, M.T.; Mawhinney, T.P.; Ganjam, I.K.; Fales, W.H.
    Characterization of a unique class C acid phosphatase from Clostridium perfringens (2009), Appl. Environ. Microbiol., 75, 3745-3754.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
His-tagged version expressed in Escherichia coli BL21 AI Clostridium perfringens

Protein Variants

Protein Variants Comment Organism
D100A 1% wild-type activity Clostridium perfringens

Inhibitors

Inhibitors Comment Organism Structure
Cd2+
-
Clostridium perfringens
Ce3+
-
Clostridium perfringens
guanosine
-
Clostridium perfringens
Hg2+
-
Clostridium perfringens
molybdate
-
Clostridium perfringens
NaF
-
Clostridium perfringens
Pb2+
-
Clostridium perfringens
ribose
-
Clostridium perfringens
Sm2+
-
Clostridium perfringens
Tartrate
-
Clostridium perfringens
vanadate
-
Clostridium perfringens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.221
-
5'-UMP
-
Clostridium perfringens
0.222
-
5'-TMP
-
Clostridium perfringens
0.269
-
5'-AMP
-
Clostridium perfringens
0.283
-
5'-GMP
-
Clostridium perfringens
0.658
-
p-nitrophenyl phosphate
-
Clostridium perfringens

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+
-
Clostridium perfringens
Cr2+
-
Clostridium perfringens
Cu2+
-
Clostridium perfringens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31000
-
2 * 31000, analytical ultracentrifugation, gel filtration, SDS-PAGE Clostridium perfringens

Organism

Organism UniProt Comment Textmining
Clostridium perfringens B1Q002 ATCC 10543
-

Purification (Commentary)

Purification (Comment) Organism
immobilized metal ion affinity chromatography (Ni2+), anion-exchange chromatography Clostridium perfringens

Renatured (Commentary)

Renatured (Comment) Organism
-
Clostridium perfringens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
46.5
-
substrate 5'-AMP Clostridium perfringens
47.4
-
substrate 5'-TMP Clostridium perfringens
52.1
-
substrate 5'-GMP Clostridium perfringens
71.4
-
substrate 5'-UMP Clostridium perfringens
98.3
-
substrate p-nitrophenyl phosphate Clostridium perfringens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-TMP + H2O
-
Clostridium perfringens thymidine + phosphate
-
?
4-methylumbelliferyl phosphate + H2O
-
Clostridium perfringens 4-methylumbelliferol + phosphate
-
?
5'-AMP + H2O
-
Clostridium perfringens adenosine + phosphate
-
?
5'-GMP + H2O
-
Clostridium perfringens guanosine + phosphate
-
?
5'-TMP + H2O
-
Clostridium perfringens thymidine + phosphate
-
?
5'-UMP + H2O
-
Clostridium perfringens uridine + phosphate
-
?
p-nitrophenyl phosphate + H2O further substrates: phenolphthalein bisphosphate, phenolphthalein monophosphate Clostridium perfringens p-nitrophenol + phosphate
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 31000, analytical ultracentrifugation, gel filtration, SDS-PAGE Clostridium perfringens

Synonyms

Synonyms Comment Organism
class C acid phosphatase
-
Clostridium perfringens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
substrate p-nitrophenyl phosphate Clostridium perfringens
6
-
substrate 5'-UMP Clostridium perfringens

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
additional information
-
80% inhibition at 1 mM Clostridium perfringens Pb2+
0.0012
-
-
Clostridium perfringens Hg2+
0.5
-
-
Clostridium perfringens NaF