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Literature summary for 3.1.3.2 extracted from

  • Veljanovski, V.; Vanderbeld, B.; Knowles, V.L.; Snedden, W.A.; Plaxton, W.C.
    Biochemical and molecular characterization of AtPAP26, a vacuolar purple acid phosphatase up-regulated in phosphate-deprived Arabidopsis suspension cells and seedlings (2006), Plant Physiol., 142, 1282-1293.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.1.3.2

Inhibitors

Inhibitors Comment Organism Structure
arsenate 8% residual activity at 5 mM Arabidopsis thaliana
Cu2+ 2% residual activity at 5 mM Arabidopsis thaliana
Fe2+ complete inhibition at 5 mM Arabidopsis thaliana
molybdate 7% residual activity at 5 mM Arabidopsis thaliana
NaF 53% residual activity at 5 mM Arabidopsis thaliana
phosphate 31% residual activity at 5 mM Arabidopsis thaliana
vanadate complete inhibition at 5 mM Arabidopsis thaliana
Zn2+ 10% residual activity at 5 mM Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole
-
 Arabidopsis thaliana 5773
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ 21% increase of activity at 5 mM Arabidopsis thaliana
Co2+ 47% increase of activity at 5 mM Arabidopsis thaliana
Mg2+ preferred divalent cation activator, 90% increase of activity at 5 mM Arabidopsis thaliana
Mn2+ 44% increase of activity at 5 mM Arabidopsis thaliana
additional information no effect on APase activity when the reaction mixture (lacking Mg2+) contains 5 mM KCl or 5 mM EDTA Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51000
-
 2 * 51000, predicted size of the mature polypeptide Arabidopsis thaliana
55000
-
 2 * 55000, gel filtration Arabidopsis thaliana
102000
-
 predicted size of the mature polypeptide Arabidopsis thaliana
110000
-
 gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q949Y3
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein
-
 Arabidopsis thaliana

Purification (Commentary)

Purification (Comment) Organism
SO3- Fractogel EMD-650 column chromatography Arabidopsis thaliana

Source Tissue

Source Tissue Comment Organism Textmining
root
-
 Arabidopsis thaliana
-
shoot
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.44
-
 clarified extract, using phosphoenolpyruvate as a substrate Arabidopsis thaliana
421
-
 after 957fold purification, using phosphoenolpyruvate as a substrate Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
AMP + H2O 9% activity compared to phosphoenolpyruvate Arabidopsis thaliana adenosine + phosphate
-
 ?
ATP + H2O 17% activity compared to phosphoenolpyruvate Arabidopsis thaliana ADP + phosphate
-
 ?
D-fructose 6-phosphate + H2O 19% activity compared to phosphoenolpyruvate Arabidopsis thaliana D-fructose + phosphate
-
 ?
D-glucose 6-phosphate + H2O 46% activity compared to phosphoenolpyruvate Arabidopsis thaliana D-glucose + phosphate
-
 ?
D-ribose 5-phosphate + H2O 40% activity compared to phosphoenolpyruvate Arabidopsis thaliana D-ribose + phosphate
-
 ?
dAMP + H2O 16% activity compared to phosphoenolpyruvate Arabidopsis thaliana 2'-deoxyadenosine + phosphate
-
 ?
diphosphate + H2O 86% activity compared to phosphoenolpyruvate Arabidopsis thaliana 2 phosphate
-
 ?
GTP + H2O 16% activity compared to phosphoenolpyruvate Arabidopsis thaliana GDP + phosphate
-
 ?
additional information no activity with phytic acid, phosphocholine, or bis-p-nitrophenyl phosphate (5 mM each) Arabidopsis thaliana ?
-
 ?
NADP+ + H2O 55% activity compared to phosphoenolpyruvate Arabidopsis thaliana NAD+ + phosphate
-
 ?
p-nitrophenyl phosphate + H2O 89% activity compared to phosphoenolpyruvate Arabidopsis thaliana p-nitrophenol + phosphate
-
 ?
phenylphosphate + H2O 100% activity compared to phosphoenolpyruvate Arabidopsis thaliana phenol + phosphate
-
 ?
phosphoenolpyruvate + H2O 100% activity Arabidopsis thaliana pyruvate + phosphate
-
 ?
phosphothreonine + H2O 39% activity compared to phosphoenolpyruvate Arabidopsis thaliana threonine + phosphate
-
 ?
phosphotyrosine + H2O 51% activity compared to phosphoenolpyruvate Arabidopsis thaliana tyrosine + phosphate
-
 ?

Subunits

Subunits Comment Organism
homodimer 2 * 51000, predicted size of the mature polypeptide Arabidopsis thaliana
homodimer 2 * 55000, gel filtration Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
APase
-
 Arabidopsis thaliana
PAP26
-
 Arabidopsis thaliana
purple acid phosphatase
-
 Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
 Arabidopsis thaliana

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.7 6.6 half-maximal activity is obtained at pH 4.7 and 6.6 Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.1
-
 in 50 mM sodium acetate, pH 5.6, 5 mM phosphoenolpyruvate, 10 mM MgCl2, 0.2 mM NADH, and 3 units of desalted rabbit muscle lactate dehydrogenase Arabidopsis thaliana phosphate