KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.95 | - |
4-nitrophenyl phosphate | at pH 2.1 and 30°C | Aspergillus niger |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger 307 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Sephadex G-100 gel filtration, DEAE-Sepharose CL 6B column chromatography, and CM-Sepharose CL 6B column chromatography | Aspergillus niger |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
9.66 | - |
culture liquide | Aspergillus niger |
361.3 | - |
after 37.5fold purification | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | 163% activity compared to 4-nitrophenyl phosphate | Aspergillus niger | 1-naphthol + phosphate | - |
? | |
1-naphthyl phosphate + H2O | 163% activity compared to 4-nitrophenyl phosphate | Aspergillus niger 307 | 1-naphthol + phosphate | - |
? | |
2-phosphoenolpyruvate + H2O | 75.6% activity compared to 4-nitrophenyl phosphate | Aspergillus niger | enolpyruvate + phosphate | - |
? | |
2-phosphoglycerate + H2O | 80.9% activity compared to 4-nitrophenyl phosphate | Aspergillus niger | glycerate + phosphate | - |
? | |
2-phosphoglycerate + H2O | 80.9% activity compared to 4-nitrophenyl phosphate | Aspergillus niger 307 | glycerate + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | 100% activity | Aspergillus niger | 4-nitrophenol + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | 100% activity | Aspergillus niger 307 | 4-nitrophenol + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | 85.2% activity compared to 4-nitrophenyl phosphate | Aspergillus niger | D-glucose + phosphate | - |
? | |
D-glucose 6-phosphate + H2O | 85.2% activity compared to 4-nitrophenyl phosphate | Aspergillus niger 307 | D-glucose + phosphate | - |
? | |
dodecasodium phytate + H2O | 245% activity compared to 4-nitrophenyl phosphate | Aspergillus niger | ? + phosphate | - |
? | |
NADP+ + H2O | 129% activity compared to 4-nitrophenyl phosphate | Aspergillus niger | NAD+ + phosphate | - |
? | |
NADP+ + H2O | 129% activity compared to 4-nitrophenyl phosphate | Aspergillus niger 307 | NAD+ + phosphate | - |
? | |
phenylphosphate + H2O | 134% activity compared to 4-nitrophenyl phosphate | Aspergillus niger | phenol + phosphate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
pH 2.1 optimum acid phosphatase | - |
Aspergillus niger |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
66 | - |
- |
Aspergillus niger |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
2.1 | - |
- |
Aspergillus niger |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 2.4 | - |
Aspergillus niger |