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Literature summary for 3.1.3.2 extracted from

  • Reilly, T.J.; Felts, R.L.; Henzl, M.T.; Calcutt, M.J.; Tanner, J.J.
    Characterization of recombinant Francisella tularensis acid phosphatase A (2006), Protein Expr. Purif., 45, 132-141.
    View publication on PubMed
Search for more literature for 3.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
gene acpA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strains DH5alpha, DH10B, and BL21(DE3), optimization of recombinant enzyme production Francisella tularensis

Inhibitors

Inhibitors Comment Organism Structure
molybdate strong inhibition Francisella tularensis
tungstate strong inhibition Francisella tularensis
vanadate strong inhibition Francisella tularensis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
additional information
-
-
 Francisella tularensis
57000
-
 x * 57000, recombinant enzyme, SDS-PAGE Francisella tularensis
136000
-
 analytical ultrafiltration and gel filtration Francisella tularensis

Organism

Organism UniProt Comment Textmining
Francisella tularensis
-
 human pathogen causing tularemia, gene acpA
-

Purification (Commentary)

Purification (Comment) Organism
12.2fold of recombinant from Escherichia coli strain BL21(DE3) by cation exchange and nickel chelate affinity chromatography Francisella tularensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0002
-
 purified recombinant enzyme Francisella tularensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-GMP + H2O
-
 Francisella tularensis guanosine + phosphate
-
 ?
3'-TMP + H2O
-
 Francisella tularensis thymidine + phosphate
-
 ?
4-methylumbelliferyl phosphate + H2O
-
 Francisella tularensis 4-methylumbelliferol + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Francisella tularensis 4-nitrophenol + phosphate
-
 ?
4-nitrophenyl phosphorylcholine + H2O at pH 7.4,synthetic substrate Francisella tularensis 4-nitrophenyl choline + phosphate
-
 ?
5'-GMP + H2O
-
 Francisella tularensis guanosine + phosphate
-
 ?
5'-UMP + H2O
-
 Francisella tularensis uridine + phosphate
-
 ?
ADP + H2O low activity Francisella tularensis AMP + phosphate
-
 ?
AMP + H2O
-
 Francisella tularensis adenosine + phosphate
-
 ?
ATP + H2O
-
 Francisella tularensis ADP + phosphate
-
 ?
D-fructose 1,6-bisphosphate + H2O
-
 Francisella tularensis ? + phosphate
-
 ?
additional information substrate specificity Francisella tularensis ?
-
 ?
NADP+ + H2O
-
 Francisella tularensis NAD+ + phosphate
-
 ?
phospho-L-threonine + H2O
-
 Francisella tularensis L-threonine + phosphate
-
 ?
phospho-L-tyrosine + H2O
-
 Francisella tularensis L-tyrosine + phosphate
-
 ?
phosphoenolpyruvate + H2O
-
 Francisella tularensis pyruvate + phosphate
-
 ?

Subunits

Subunits Comment Organism
oligomer x * 57000, recombinant enzyme, SDS-PAGE Francisella tularensis

Synonyms

Synonyms Comment Organism
acid phosphatase A
-
 Francisella tularensis
AcpA
-
 Francisella tularensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Francisella tularensis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
 the enzyme is heat-labile Francisella tularensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.9 6.1
-
 Francisella tularensis