Inhibitors | Comment | Organism | Structure |
---|---|---|---|
KH2PO4 | intra- and extracellular enzyme forms | Aspergillus caespitosus | |
additional information | intra- and extracellular enzyme forms are both resistant to tartrate | Aspergillus caespitosus | |
NaF | inhibition of the intracellular enzyme, only slight inhibition of the extracellular enzyme | Aspergillus caespitosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.029 | - |
4-nitrophenyl phosphate | pH 5.6, 80°C, intracellular enzyme | Aspergillus caespitosus | |
0.082 | - |
4-nitrophenyl phosphate | pH 5.6, 80°C, extracellular enzyme | Aspergillus caespitosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Aspergillus caespitosus | - |
- |
intracellular | - |
Aspergillus caespitosus | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
74000 | - |
2 * 84000, extracellular enzyme form, SDS-PAGE, 2 * 74000, intracellular enzyme form, SDS-PAGE | Aspergillus caespitosus |
84000 | - |
2 * 84000, extracellular enzyme form, SDS-PAGE, 2 * 74000, intracellular enzyme form, SDS-PAGE | Aspergillus caespitosus |
186000 | - |
extracellular enzyme form, gel filtration | Aspergillus caespitosus |
190000 | - |
intracellular enzyme form, gel filtration | Aspergillus caespitosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus caespitosus | - |
intra- and extracellular enzyme forms | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | both intra- and extracellular enzyme forms are glycoproteins showing 58.3% and 63.0% carbohydrate content, respectively | Aspergillus caespitosus |
Purification (Comment) | Organism |
---|---|
separation of enzyme forms by ion exchange chromatography and concanavalin A affinity chromatography | Aspergillus caespitosus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | optimal culture conditions for enzyme production are 40°C and pH 6.0 | Aspergillus caespitosus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | higher activity with extracellular enzyme | Aspergillus caespitosus | 4-nitrophenol + phosphate | - |
? | |
additional information | intra- and extracellular enzyme forms show also phytase activity | Aspergillus caespitosus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 84000, extracellular enzyme form, SDS-PAGE, 2 * 74000, intracellular enzyme form, SDS-PAGE | Aspergillus caespitosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
intra- and extracellular enzyme forms | Aspergillus caespitosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
stable for over 60 min, intra- and extracellular enzyme forms | Aspergillus caespitosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
intra- and extracellular enzyme forms | Aspergillus caespitosus |