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Literature summary for 3.1.3.2 extracted from

  • Passariello, C.; Forleo, C.; Micheli, V.; Schippa, S.; Leone, R.; Mangani, S.; Thaller, M.C.; Rossolini, G.M.
    Biochemical characterization of the class B acid phosphatase (AphA) of Escherichia coli MG1655 (2006), Biochim. Biophys. Acta, 1764, 13-19.
    View publication on PubMed
Search for more literature for 3.1.3.2

Cloned(Commentary)

Cloned (Comment) Organism
gene aphA, overexpression in strain DH5alpha Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
1,10-phenanthroline IC50 is 0.0020 mM Escherichia coli
dipicolinic acid IC50 is 0.0025 mM Escherichia coli
EDTA IC50 is 0.0004 mM Escherichia coli
EGTA IC50 is 0.0025 mM Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics Escherichia coli
0.0013
-
 3'-dCMP pH 6.0, 37°C, recombinant AphA Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0AE22 gene aphA
-
Escherichia coli MG1655 P0AE22 gene aphA
-

Purification (Commentary)

Purification (Comment) Organism
recombinant AphA from strain DH5alpha Escherichia coli

Reaction

Reaction Comment Organism Reaction ID
a phosphate monoester + H2O = an alcohol + phosphate modelling of substrate binding in the active site Escherichia coli
a

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3'-AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
3'-AMP + H2O
-
 Escherichia coli MG1655 adenosine + phosphate
-
 ?
3'-CMP + H2O
-
 Escherichia coli cytidine + phosphate
-
 ?
3'-dAMP + H2O
-
 Escherichia coli 3'-deoxyadenosine + phosphate
-
 ?
3'-dCMP + H2O best substrate Escherichia coli deoxycytidine + phosphate
-
 ?
3'-dGMP + H2O
-
 Escherichia coli deoxyguanosine + phosphate
-
 ?
3'-dUMP + H2O
-
 Escherichia coli deoxyuridine + phosphate
-
 ?
3'-GMP + H2O
-
 Escherichia coli guanosine + phosphate
-
 ?
3'-UMP + H2O
-
 Escherichia coli uridine + phosphate
-
 ?
3-phosphoglycerate + H2O low activity Escherichia coli glycerate + phosphate
-
 ?
3-phosphoglycerate + H2O low activity Escherichia coli MG1655 glycerate + phosphate
-
 ?
4-nitrophenyl phosphate + H2O
-
 Escherichia coli 4-nitrophenol + phosphate
-
 ?
5'-AMP + H2O
-
 Escherichia coli adenosine + phosphate
-
 ?
5'-AMP + H2O
-
 Escherichia coli MG1655 adenosine + phosphate
-
 ?
5'-CMP + H2O
-
 Escherichia coli cytidine + phosphate
-
 ?
5'-dAMP + H2O
-
 Escherichia coli deoxyadenosine + phosphate
-
 ?
5'-dCMP + H2O
-
 Escherichia coli deoxycytidine + phosphate
-
 ?
5'-dGMP + H2O
-
 Escherichia coli deoxyguanosine + phosphate
-
 ?
5'-dUMP + H2O
-
 Escherichia coli deoxyuridine + phosphate
-
 ?
5'-GMP + H2O
-
 Escherichia coli guanosine + phosphate
-
 ?
5'-UMP + H2O
-
 Escherichia coli uridine + phosphate
-
 ?
ADP + H2O
-
 Escherichia coli AMP + phosphate
-
 ?
ATP + H2O
-
 Escherichia coli ADP + phosphate
-
 ?
D-glucose 6-phosphate + H2O low activity Escherichia coli D-glucose + phosphate
-
 ?
D-ribose 5-phosphate + H2O low activity Escherichia coli D-ribose + phosphate
-
 ?
D-ribose 5-phosphate + H2O low activity Escherichia coli MG1655 D-ribose + phosphate
-
 ?
additional information substrate specificity of AphA, overview Escherichia coli ?
-
 ?
additional information substrate specificity of AphA, overview Escherichia coli MG1655 ?
-
 ?

Synonyms

Synonyms Comment Organism
AphA
-
 Escherichia coli
More the enzyme belongs to the class B bacterial phosphatases as part of the DDDD superfamily of phosphohydrolases Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Escherichia coli

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Escherichia coli
101
-
 3'-dCMP pH 6.0, 37°C, recombinant AphA Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 assay at Escherichia coli

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.0004
-
 IC50 is 0.0004 mM Escherichia coli EDTA
0.002
-
 IC50 is 0.0020 mM Escherichia coli 1,10-phenanthroline
0.0025
-
 IC50 is 0.0025 mM Escherichia coli EGTA
0.0025
-
 IC50 is 0.0025 mM Escherichia coli dipicolinic acid