Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 2fold activation of kcat, 14fold activition combined with trypsin cleavage | Homo sapiens | |
L-ascorbic acid | 3fold activation of kcat | Homo sapiens | |
Trypsin | 2fold activation of kcat by trypsin cleavage, 14fold activition combined with 2-mercaptoethanol addition | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of TRACP in Spodopetra frugiperda Sf9 cells using the baculovirus infection system | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme is tartrate-resistant | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.3 | - |
4-nitrophenyl phosphate | pH 6.2-6.4, 37°C, recombinant enzyme, in presence of L-ascorbate | Homo sapiens | |
2.6 | - |
4-nitrophenyl phosphate | pH 6.2-6.4, 37°C, trypsin-cleaved recombinant enzyme, in presence of L-ascorbate | Homo sapiens | |
4.7 | - |
4-nitrophenyl phosphate | pH 6.2-6.4, 37°C, trypsin-cleaved recombinant enzyme | Homo sapiens | |
5.6 | - |
4-nitrophenyl phosphate | pH 5.4-5.6, 37°C, recombinant enzyme | Homo sapiens | |
8.7 | - |
4-nitrophenyl phosphate | pH 6.2-6.4, 37°C, trypsin-cleaved recombinant enzyme, in presence of 2-mercaptoethanol | Homo sapiens | |
11.6 | - |
4-nitrophenyl phosphate | pH 5.4-5.6, 37°C, recombinant enzyme, in presence of 2-mercaptoethanol | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme 84fold from Sf9 insect cells by cation exchange chromatography and 2-step gel filtration | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
92.1 | - |
purified recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl phosphate + H2O | - |
Homo sapiens | 4-nitrophenol + phosphate | - |
? | |
additional information | the enzyme also shows ROS-generating activity | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
purple acid phosphatase | - |
Homo sapiens |
tartrate-resistant acid phosphatase | - |
Homo sapiens |
TRACP | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14.1 | - |
4-nitrophenyl phosphate | pH 5.4-5.6, 37°C, recombinant enzyme | Homo sapiens | |
23.8 | - |
4-nitrophenyl phosphate | pH 6.2-6.4, 37°C, trypsin-cleaved recombinant enzyme, in presence of L-ascorbate | Homo sapiens | |
26.4 | - |
4-nitrophenyl phosphate | pH 6.2-6.4, 37°C, trypsin-cleaved recombinant enzyme | Homo sapiens | |
30.4 | - |
4-nitrophenyl phosphate | pH 5.4-5.6, 37°C, recombinant enzyme, in presence of 2-mercaptoethanol | Homo sapiens | |
38.2 | - |
4-nitrophenyl phosphate | pH 6.2-6.4, 37°C, recombinant enzyme, in presence of L-ascorbate | Homo sapiens | |
88.6 | - |
4-nitrophenyl phosphate | pH 6.2-6.4, 37°C, trypsin-cleaved recombinant enzyme, in presence of L-ascorbate | Homo sapiens | |
200.4 | - |
4-nitrophenyl phosphate | pH 6.2-6.4, 37°C, trypsin-cleaved recombinant enzyme, in presence of 2-mercaptoethanol | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.4 | 5.6 | - |
Homo sapiens |
6.2 | 6.4 | pH optimum is shifted in presence of ascorbate or by trypsin cleavage | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.8 | 6.6 | - |
Homo sapiens |