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Literature summary for 3.1.3.2 extracted from

  • Fagerlund, K.M.; Ylipahkala, H.; Tiitinen, S.L.; Janckila, A.J.; Hamilton, S.; Maentausta, O.; Vaananen, H.K.; Halleen, J.M.
    Effects of proteolysis and reduction on phosphatase and ROS-generating activity of human tartrate-resistant acid phosphatase (2006), Arch. Biochem. Biophys., 449, 1-7.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 2fold activation of kcat, 14fold activition combined with trypsin cleavage Homo sapiens
L-ascorbic acid 3fold activation of kcat Homo sapiens
Trypsin 2fold activation of kcat by trypsin cleavage, 14fold activition combined with 2-mercaptoethanol addition Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of TRACP in Spodopetra frugiperda Sf9 cells using the baculovirus infection system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is tartrate-resistant Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3
-
4-nitrophenyl phosphate pH 6.2-6.4, 37°C, recombinant enzyme, in presence of L-ascorbate Homo sapiens
2.6
-
4-nitrophenyl phosphate pH 6.2-6.4, 37°C, trypsin-cleaved recombinant enzyme, in presence of L-ascorbate Homo sapiens
4.7
-
4-nitrophenyl phosphate pH 6.2-6.4, 37°C, trypsin-cleaved recombinant enzyme Homo sapiens
5.6
-
4-nitrophenyl phosphate pH 5.4-5.6, 37°C, recombinant enzyme Homo sapiens
8.7
-
4-nitrophenyl phosphate pH 6.2-6.4, 37°C, trypsin-cleaved recombinant enzyme, in presence of 2-mercaptoethanol Homo sapiens
11.6
-
4-nitrophenyl phosphate pH 5.4-5.6, 37°C, recombinant enzyme, in presence of 2-mercaptoethanol Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme 84fold from Sf9 insect cells by cation exchange chromatography and 2-step gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Homo sapiens
92.1
-
purified recombinant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
Homo sapiens 4-nitrophenol + phosphate
-
?
additional information the enzyme also shows ROS-generating activity Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
purple acid phosphatase
-
Homo sapiens
tartrate-resistant acid phosphatase
-
Homo sapiens
TRACP
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14.1
-
4-nitrophenyl phosphate pH 5.4-5.6, 37°C, recombinant enzyme Homo sapiens
23.8
-
4-nitrophenyl phosphate pH 6.2-6.4, 37°C, trypsin-cleaved recombinant enzyme, in presence of L-ascorbate Homo sapiens
26.4
-
4-nitrophenyl phosphate pH 6.2-6.4, 37°C, trypsin-cleaved recombinant enzyme Homo sapiens
30.4
-
4-nitrophenyl phosphate pH 5.4-5.6, 37°C, recombinant enzyme, in presence of 2-mercaptoethanol Homo sapiens
38.2
-
4-nitrophenyl phosphate pH 6.2-6.4, 37°C, recombinant enzyme, in presence of L-ascorbate Homo sapiens
88.6
-
4-nitrophenyl phosphate pH 6.2-6.4, 37°C, trypsin-cleaved recombinant enzyme, in presence of L-ascorbate Homo sapiens
200.4
-
4-nitrophenyl phosphate pH 6.2-6.4, 37°C, trypsin-cleaved recombinant enzyme, in presence of 2-mercaptoethanol Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4 5.6
-
Homo sapiens
6.2 6.4 pH optimum is shifted in presence of ascorbate or by trypsin cleavage Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
4.8 6.6
-
Homo sapiens