Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 5 mM, SAP2, 50% inhibition | Solanum lycopersicum | |
Ca2+ | 5 mM, SAP2, 60% inhibition | Solanum lycopersicum | |
Co2+ | 5 mM, SAP2, 95% inhibition | Solanum lycopersicum | |
Cu2+ | 5 mM, SAP1, 91% inhibition | Solanum lycopersicum | |
EDTA | 5 mM, SAP2, 71% inhibition | Solanum lycopersicum | |
F- | - |
Solanum lycopersicum | |
Mn2+ | 5 mM, SAP2, 26% inhibition | Solanum lycopersicum | |
molybdate | competitive | Solanum lycopersicum | |
additional information | SAP1 and SAP2 are not inhibited by tartrate | Solanum lycopersicum | |
phosphate | mixed and competitive | Solanum lycopersicum | |
Zn2+ | 5 mM, SAP2, complete inhibition of SAP1 and SAP2 | Solanum lycopersicum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.4 | - |
phosphoenolpyruvate | pH 5.3, 25°C, SAP2 | Solanum lycopersicum | |
1.9 | - |
Tetrapolyphosphate | pH 5.3, 25°C, SAP1 | Solanum lycopersicum | |
2.1 | - |
phosphoenolpyruvate | pH 5.3, 25°C, SAP1 | Solanum lycopersicum | |
2.1 | - |
Phenyl phosphate | pH 5.3, 25°C, SAP1 | Solanum lycopersicum | |
2.1 | - |
phosphotyrosine | pH 5.3, 25°C, SAP2 | Solanum lycopersicum | |
2.9 | - |
Tetrapolyphosphate | pH 5.3, 25°C, SAP2 | Solanum lycopersicum | |
3.3 | - |
4-nitrophenyl phosphate | pH 5.3, 25°C, SAP2 | Solanum lycopersicum | |
3.9 | - |
2-naphthyl phosphate | pH 5.3, 25°C, SAP1 | Solanum lycopersicum | |
4.1 | - |
ATP | pH 5.3, 25°C, SAP1 | Solanum lycopersicum | |
4.5 | - |
6-phospho-D-gluconate | - |
Solanum lycopersicum | |
4.5 | - |
4-nitrophenyl phosphate | pH 5.3, 25°C, SAP1 | Solanum lycopersicum | |
4.5 | - |
2-naphthyl phosphate | pH 5.3, 25°C, SAP2 | Solanum lycopersicum | |
4.6 | - |
diphosphate | pH 5.3, 25°C, SAP2 | Solanum lycopersicum | |
5 | - |
glycerol 3-phosphate | pH 5.3, 25°C, SAP2 | Solanum lycopersicum | |
5.3 | - |
Phenyl phosphate | pH 5.3, 25°C, SAP2 | Solanum lycopersicum | |
5.3 | - |
1-naphthyl phosphate | pH 5.3, 25°C, SAP2 | Solanum lycopersicum | |
5.4 | - |
GTP | pH 5.3, 25°C, SAP2 | Solanum lycopersicum | |
5.5 | - |
ATP | pH 5.3, 25°C, SAP2 | Solanum lycopersicum | |
6 | - |
3-phosphoglycerate | pH 5.3, 25°C, SAP2 | Solanum lycopersicum | |
6.4 | - |
1-naphthyl phosphate | pH 5.3, 25°C, SAP1 | Solanum lycopersicum | |
8.6 | - |
GTP | pH 5.3, 25°C, SAP1 | Solanum lycopersicum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Solanum lycopersicum | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 5 mM, SAP1 is activated to 115% of control | Solanum lycopersicum | |
EDTA | 5 mM, SAP1 is activated to 114% of control | Solanum lycopersicum | |
Mg2+ | 5 mM, SAP1 is activated to 136% of control, SAP2 is activated to 181% of control | Solanum lycopersicum | |
Mn2+ | 5 mM, SAP1 is activated to 113% of control | Solanum lycopersicum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum lycopersicum | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | - |
Solanum lycopersicum |
Purification (Comment) | Organism |
---|---|
two secreted acid phosphatases: SAP1 and SAP2 | Solanum lycopersicum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell suspension culture | - |
Solanum lycopersicum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
246 | - |
SAP1 | Solanum lycopersicum |
940 | - |
SAP2 | Solanum lycopersicum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | reaction with SAP1 and SAP2 | Solanum lycopersicum | 1-naphthol + phosphate | - |
? | |
2-naphthyl phosphate + H2O | reaction with SAP1 and SAP2 | Solanum lycopersicum | 2-naphthol + phosphate | - |
? | |
3-phosphoglycerate + H2O | reaction with SAP2, no activity with SAP1 | Solanum lycopersicum | glycerate + phosphate | - |
? | |
4-nitrophenyl phosphate + H2O | reaction with SAP1 and SAP2 | Solanum lycopersicum | 4-nitrophenol + phosphate | - |
? | |
6-phospho-D-gluconate + H2O | reaction with SAP2, no activity with SAP1 | Solanum lycopersicum | D-gluconate + phosphate | - |
? | |
ATP + H2O | reaction with SAP1 and SAP2 | Solanum lycopersicum | ? | - |
? | |
diphosphate + H2O | reaction with SAP2, no activity with SAP1 | Solanum lycopersicum | 2 phosphate | - |
? | |
glycerol 3-phosphate + H2O | reaction with SAP2, no activity with SAP1 | Solanum lycopersicum | glycerol + phosphate | - |
? | |
GTP + H2O | reaction with SAP1 and SAP2 | Solanum lycopersicum | ? | - |
? | |
additional information | SAP1 and SAP2 are multifunctional enzymes, the enzymes also exhibit significant peroxidase activity which is optimal at approximately pH 8.4 and insensitive to Mg2+ or molybdate | Solanum lycopersicum | ? | - |
? | |
phenyl phosphate + H2O | reaction with SAP1 and SAP2 | Solanum lycopersicum | phenol + phosphate | - |
? | |
phosphoenolpyruvate + H2O | reaction with SAP1 and SAP2 | Solanum lycopersicum | pyruvate + phosphate | - |
? | |
phosphotyrosine + H2O | reaction with SAP2, no activity with SAP1 | Solanum lycopersicum | tyrosine + phosphate | - |
? | |
tetrapolyphosphate + H2O | reaction with SAP1 and SAP2 | Solanum lycopersicum | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
purple acid phosphatase | - |
Solanum lycopersicum |
SAP1 | - |
Solanum lycopersicum |
SAP2 | - |
Solanum lycopersicum |
secreted acid phosphatase | - |
Solanum lycopersicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.3 | - |
isoenzyme SAP1 and SAP2 | Solanum lycopersicum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0002 | - |
molybdate | pH 5.3, 25°C, SAP1 or SAP2 | Solanum lycopersicum | |
0.48 | - |
F- | pH 5.3, 25°C, SAP1 | Solanum lycopersicum | |
0.95 | - |
F- | pH 5.3, 25°C, SAP1 | Solanum lycopersicum | |
1.2 | - |
phosphate | pH 5.3, 25°C, SAP1 | Solanum lycopersicum | |
1.3 | - |
phosphate | pH 5.3, 25°C, SAP1 | Solanum lycopersicum |