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Literature summary for 3.1.3.2 extracted from

  • Wannet, W.J.B.; Wassenaar, R.W.; Jorissen, H.J.M.M.; Van der Drift, C.; Op Den Camp, H.J.M.
    Purification and characterization of an acid phosphatase from the commercial mushroom Agaricus bisporus (2000), Antonie van Leeuwenhoek, 77, 215-222.
    View publication on PubMed
Search for more literature for 3.1.3.2

General Stability

General Stability Organism
to minimize activity loss during the purification procedure the best stabilizing results are obtained by addition of a combination of 1 mM DTT, 1 mM EDTA and glycerol Agaricus bisporus

Inhibitors

Inhibitors Comment Organism Structure
additional information unaffected by EDTA Agaricus bisporus
phosphate
-
 Agaricus bisporus
Tartrate
-
 Agaricus bisporus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0031
-
 D-fructose 6-phosphate pH 4.8, 30°C Agaricus bisporus
0.37
-
 4-nitrophenyl phosphate pH 4.8, 30°C Agaricus bisporus

Localization

Localization Comment Organism GeneOntology No. Textmining
vacuole
-
 Agaricus bisporus 5773
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39000
-
 4 * 39000, SDS-PAGE Agaricus bisporus
145000
-
 non-denaturing PAGE Agaricus bisporus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Agaricus bisporus key enzyme involved in the biosynthesis of mannitol ?
-
 ?

Organism

Organism UniProt Comment Textmining
Agaricus bisporus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein maximal carbohydrate content of 30% Agaricus bisporus

Purification (Commentary)

Purification (Comment) Organism
-
 Agaricus bisporus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
225
-
-
 Agaricus bisporus

Storage Stability

Storage Stability Organism
-20°C, enzyme in cell-free extract remains stable for 6 months Agaricus bisporus
4°C, enzyme in cell-free extract remains stable for at least 8 days Agaricus bisporus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl phosphate + H2O
-
 Agaricus bisporus 4-nitrophenol + phosphate
-
 ?
AMP + H2O
-
 Agaricus bisporus adenosine + phosphate
-
 ?
beta-glycerol phosphate + H2O
-
 Agaricus bisporus glycerol + phosphate
-
 ?
D-fructose 6-phosphate + H2O
-
 Agaricus bisporus D-fructose + phosphate
-
 ?
D-glucose 6-phosphate + H2O
-
 Agaricus bisporus D-glucose + phosphate
-
 ?
D-mannitol 6-phosphate + H2O
-
 Agaricus bisporus D-mannitol + phosphate
-
 ?
D-ribose 5-phosphate + H2O
-
 Agaricus bisporus D-ribose + phosphate
-
 ?
additional information no activity is observed with fructose 1,6-diphopshate, glucose 1-phosphate, ATP, ADP and acetyl phosphate Agaricus bisporus ?
-
 ?
additional information key enzyme involved in the biosynthesis of mannitol Agaricus bisporus ?
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 39000, SDS-PAGE Agaricus bisporus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
 D-fructose 6-phosphate as substrate Agaricus bisporus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
40 70 40°C: about 60% of maximal activity, 70°C: about 70% of maximal activity, D-fructose 6-phosphate as substrate Agaricus bisporus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
 D-fructose 6-phosphate as substrate Agaricus bisporus

pH Range

pH Minimum pH Maximum Comment Organism
4 5.5 pH 2.0: no activity, pH 4.0: about 90% of maximal activity, pH 5.5: about 60% of maximal activity, D-fructose 6-phosphate as substrate Agaricus bisporus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
5
-
 phosphate pH 4.8, 30°C Agaricus bisporus

pI Value

Organism Comment pI Value Maximum pI Value
Agaricus bisporus isoelectric focusing 4.8 4.7