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Literature summary for 3.1.3.2 extracted from
- Penicillium chrysogenum extracellular acid phosphatase: purification and biochemical characterization (1991), Biochim. Biophys. Acta, 1074, 392-397.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | - |
Penicillium chrysogenum |  |
| phosphate | competitive | Penicillium chrysogenum | |
| Phosphomycin | ,10 mM, 25% inhibition | Penicillium chrysogenum | |
| SDS | - |
Penicillium chrysogenum | |
| Zn2+ | - |
Penicillium chrysogenum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
gel filtration | Penicillium chrysogenum |
| 57000 | - |
1 * 57000, SDS-PAGE | Penicillium chrysogenum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | contains 12% carbohydrate | Penicillium chrysogenum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Penicillium chrysogenum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| culture medium | - |
Penicillium chrysogenum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 364 | - |
- |
Penicillium chrysogenum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl phosphate + H2O | - |
Penicillium chrysogenum | p-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 57000, SDS-PAGE | Penicillium chrysogenum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Penicillium chrysogenum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 10 | 60 | 10°C: 20% of maximal activity, 30°C: 45% of maximal activity, 60°C: optimum | Penicillium chrysogenum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
27% loss of activity after 5 min, 33% loss of activity after 10 min, 67% loss of activity after 15 min | Penicillium chrysogenum |
| 70 | - |
10 min, complete inactivation | Penicillium chrysogenum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Penicillium chrysogenum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 6.5 | pH 4.5: about 30% of maximal activity, pH 6.5: about 15% of maximal activity | Penicillium chrysogenum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | - |
loss of activity below | Penicillium chrysogenum |
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