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Literature summary for 3.1.3.2 extracted from

  • Olczak, M.; Watorek, W.; Morawiecka, B.
    Purification and characterization of acid phosphatase from yellow lupin (Lupinus luteus) seeds (1997), Biochim. Biophys. Acta, 1341, 14-25.
    View publication on PubMed
Search for more literature for 3.1.3.2

Inhibitors

Inhibitors Comment Organism Structure
F-
-
 Lupinus luteus
molybdate
-
 Lupinus luteus
phosphate
-
 Lupinus luteus
Zn2+ 2.0 mM, 90% loss of activity Lupinus luteus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.17
-
 1-naphthyl phosphate
-
 Lupinus luteus
0.2
-
 NADPH
-
 Lupinus luteus
0.23
-
 fructose 1,6-diphosphate
-
 Lupinus luteus
0.25
-
 o-carboxyphenyl phosphate
-
 Lupinus luteus
0.27
-
 Phenyl phosphate
-
 Lupinus luteus
0.28
-
 p-nitrophenyl phosphate
-
 Lupinus luteus
0.64
-
 3'-UMP
-
 Lupinus luteus
0.69
-
 2-phosphoglycerate
-
 Lupinus luteus
0.76
-
 3'-AMP
-
 Lupinus luteus
0.84
-
 1-phosphoglycerate
-
 Lupinus luteus
0.86
-
 phosphoenolpyruvate
-
 Lupinus luteus
0.87
-
 O-phospho-L-serine
-
 Lupinus luteus
0.9
-
 glucose 6-phosphate
-
 Lupinus luteus
1.1
-
 fructose 6-phosphate
-
 Lupinus luteus
1.18
-
 O-phospho-L-threonine
-
 Lupinus luteus
1.31
-
 ribose 5-phosphate
-
 Lupinus luteus
1.43
-
 O-phospho-L-tyrosine
-
 Lupinus luteus
4.08
-
 2'-AMP
-
 Lupinus luteus
24.4
-
 glucose 1-phosphate
-
 Lupinus luteus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 1 * 44000 + 1 * 50000, SDS-PAGE Lupinus luteus
50000
-
 1 * 44000 + 1 * 50000, SDS-PAGE Lupinus luteus
93500
-
 gel filtration Lupinus luteus

Organism

Organism UniProt Comment Textmining
Lupinus luteus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein contains 7.3% carbohydrate forming at least 4 oligosaccharide chains. One molecule of enzyme is glycosylated with 20-22 mannose, 13-14 N-acetylglucosamine, 3-5 fucose and 4 xylose residues Lupinus luteus

Purification (Commentary)

Purification (Comment) Organism
-
 Lupinus luteus

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Lupinus luteus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
895
-
-
 Lupinus luteus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-glycerate phosphate + H2O
-
 Lupinus luteus glycerate + phosphate
-
 ?
1-naphthyl phosphate + H2O
-
 Lupinus luteus 1-naphthol + phosphate
-
 ?
1-phosphoglycerate + H2O
-
 Lupinus luteus glycerate + phosphate
-
 ?
2'-AMP + H2O
-
 Lupinus luteus adenosine + phosphate
-
 ?
2-phosphoglycerate + H2O
-
 Lupinus luteus glycerate + phosphate
-
 ?
3'-AMP + H2O
-
 Lupinus luteus adenosine + phosphate
-
 ?
3'-UMP + H2O
-
 Lupinus luteus uridine + phosphate
-
 ?
D-fructose 1,6-diphosphate + H2O
-
 Lupinus luteus ?
-
 ?
D-fructose 6-phosphate + H2O
-
 Lupinus luteus D-fructose + phosphate
-
 ?
D-ribose 5-phosphate + H2O
-
 Lupinus luteus D-ribose + phosphate
-
 ?
Fructose 1-phosphate + H2O
-
 Lupinus luteus Fructose + phosphate
-
 ?
glucose 1-phosphate + H2O
-
 Lupinus luteus glucose + phosphate
-
 ?
Glucose 6-phosphate + H2O
-
 Lupinus luteus Glucose + phosphate
-
 ?
NADPH + H2O
-
 Lupinus luteus NADH + phosphate
-
 ?
o-carboxyphenyl phosphoric acid + H2O
-
 Lupinus luteus o-carboxyphenol + phosphate
-
 ?
O-phospho-L-threonine + H2O
-
 Lupinus luteus L-threonine + phosphate
-
 ?
O-phospho-L-Tyr + H2O
-
 Lupinus luteus Tyr + phosphate
-
 ?
O-phosphoserine + H2O
-
 Lupinus luteus Ser + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Lupinus luteus p-nitrophenol + phosphate
-
 ?
phenyl phosphate + H2O
-
 Lupinus luteus phenol + phosphate
-
 ?
phosphoenolpyruvate + H2O
-
 Lupinus luteus pyruvate + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 1 * 44000 + 1 * 50000, SDS-PAGE Lupinus luteus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.4
-
-
 Lupinus luteus