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Literature summary for 3.1.3.2 extracted from
- Nonspecific acid phosphatase from Schizosaccharomyces pombe. Purification and physical chemical properties (1975), Biochemistry, 14, 2847-2852.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | - |
Schizosaccharomyces pombe |  |
| SO42- | - |
Schizosaccharomyces pombe | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.17 | - |
4-nitrophenyl phosphate | - |
Schizosaccharomyces pombe | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 104000 | - |
x * 104000, enzyme dissociated in 8 M urea and 1% SDS, SDS-PAGE | Schizosaccharomyces pombe |
| 381000 | - |
gel filtration | Schizosaccharomyces pombe |
| 383000 | - |
sucrose density gradient centrifugation | Schizosaccharomyces pombe |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Schizosaccharomyces pombe | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | enzyme contains 66% neutral sugar. Mannose and galactose are main components of the carbohydrate moiety, glucosamine is present in lower amount | Schizosaccharomyces pombe |
| side-chain modification | the enzyme contains 1 mol phosphate per mol of enzyme | Schizosaccharomyces pombe |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Schizosaccharomyces pombe |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 412 | - |
- |
Schizosaccharomyces pombe |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl phosphate + H2O | - |
Schizosaccharomyces pombe | p-nitrophenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 104000, enzyme dissociated in 8 M urea and 1% SDS, SDS-PAGE | Schizosaccharomyces pombe |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
rapid inactivation above | Schizosaccharomyces pombe |
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