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Literature summary for 3.1.3.2 extracted from

  • Wojcieszyn, J.W.; Wang, M.C.; Lee, C.L.; Murphy, G.P.; Chu, T.M.
    Purification and characterization of a human urinary acid phosphatase (1979), J. Appl. Biochem., 1, 223-234.
No PubMed abstract available
Search for more literature for 3.1.3.2

Inhibitors

Inhibitors Comment Organism Structure
F-
-
 Homo sapiens
Fe2+
-
 Homo sapiens
Mg2+
-
 Homo sapiens
phosphate
-
 Homo sapiens
Tartrate
-
 Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.1
-
 Alpha-naphthyl phosphate urinary enzyme and prostatic enzyme Homo sapiens
0.36
-
 p-nitrophenyl phosphate prostatic enzyme Homo sapiens
0.52
-
 p-nitrophenyl phosphate urinary enzyme Homo sapiens
1
-
 AMP urinary enzyme Homo sapiens
2
-
 AMP prostatic enzyme Homo sapiens
3.3
-
 phosphorylcholine prostatic enzyme Homo sapiens
3.3
-
 thymolphthalein monophosphate urinary enzyme Homo sapiens
4.6
-
 phosphoryl choline urinary enzyme Homo sapiens
5
-
 thymolphthalein monophosphate prostatic enzyme Homo sapiens
45.3
-
 2-glycerophosphate prostatic enzyme Homo sapiens
66.7
-
 2-glycerophosphate urinary enzyme Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 2 * 48000, urinary enzyme, SDS-PAGE Homo sapiens
97000
-
 urinary enzyme, sucrose density gradient ultracentrifugation Homo sapiens
100000
-
 urinary enzyme, gel filtration, native gradient PAGE Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
isoenzyme A, B and C 103 Homo sapiens
urine Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
prostate gland
-
 Homo sapiens
-
urine female Homo sapiens
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
333
-
 urinary enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-naphthyl phosphate + H2O
-
 Homo sapiens 1-naphthol + phosphate
-
 ?
2-glycerophosphate + H2O
-
 Homo sapiens glycerol + phosphate
-
 ?
AMP + H2O
-
 Homo sapiens adenosine + phosphate
-
 ?
p-nitrophenyl phosphate + H2O
-
 Homo sapiens p-nitrophenol + phosphate
-
 ?
phosphocholine + H2O
-
 Homo sapiens choline + phosphate
-
 ?
thymolphthalein monophosphate + H2O
-
 Homo sapiens thymolphthalein + phosphate
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 48000, urinary enzyme, SDS-PAGE Homo sapiens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
 2 h, 39% loss of activity of urinary enzyme, 88% loss of activity of prostatic enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.8
-
 urinary enzyme Homo sapiens