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Literature summary for 3.1.3.2 extracted from
- The acid phosphatase with optimum pH of 2.5 of Escherichia coli. Physiological and biochemical study (1982), J. Biol. Chem., 257, 6669-6676.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| acetate | stimulates | Escherichia coli |  |
| citrate | stimulates | Escherichia coli | |
| pyruvate | stimulates | Escherichia coli | |
| succinate | stimulates | Escherichia coli | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| F- | - |
Escherichia coli | |
| Tartrate | - |
Escherichia coli | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.35 | - |
GTP | - |
Escherichia coli | |
| 30 | - |
ATP | - |
Escherichia coli | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Br- | slight stimulation | Escherichia coli | |
| I- | slight stimulation | Escherichia coli | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
gel filtration | Escherichia coli |
| 45000 | - |
1 * 45000, SDS-PAGE | Escherichia coli |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Escherichia coli | synthesis starts as soon as cultures enter the stationary phase, strong negative control by cAMP, starvation for inorganic phosphate results in a premature full induction | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Escherichia coli |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -18°C, 1 year, no significant modification | Escherichia coli |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + H2O | low activity | Escherichia coli | ADP + phosphate | - |
? | |
| GTP + H2O | - |
Escherichia coli | GDP + phosphate | - |
? | |
| additional information | synthesis starts as soon as cultures enter the stationary phase, strong negative control by cAMP, starvation for inorganic phosphate results in a premature full induction | Escherichia coli | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 45000, SDS-PAGE | Escherichia coli |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | - |
glycine buffer | Escherichia coli |
| 2.8 | - |
naphthylate buffer | Escherichia coli |
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