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Literature summary for 3.1.3.2 extracted from
- Purification and characterization of purple acid phosphatase from developing rat bone (1986), Arch. Biochem. Biophys., 247, 131-139.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbate | plus Fe2+, increases activity with the substrates AMP, diphosphate and phosphoproteins | Rattus norvegicus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 1 mM | Rattus norvegicus | |
| F- | 10 mM | Rattus norvegicus | |
| Fe2+ | 0.1 mM, 50% inhibition of hydrolysis of p-nitrophenyl phosphate | Rattus norvegicus | |
| molybdate | 10 mM | Rattus norvegicus | |
| Tartrate | tartrate has no effect | Rattus norvegicus | |
| Zn2+ | 1 mM | Rattus norvegicus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Fe2+ | plus scorbate, increases activity with the substrates AMP, diphosphate and phosphoproteins | Rattus norvegicus | |
| Fe2+ | stimulates dephosphorylation of ATP | Rattus norvegicus | |
| Iron | enzyme contains 1.3 atoms of iron per enzyme molecule | Rattus norvegicus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 16300 | - |
x * 16300, SDS-PAGE | Rattus norvegicus |
| 40000 | - |
gel filtration | Rattus norvegicus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rattus norvegicus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| bone | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 530 | - |
- |
Rattus norvegicus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ADP + H2O | 68% of activity with p-nitrophenyl phosphate | Rattus norvegicus | AMP + phosphate | - |
? | |
| ADP + H2O | 78% of activity with p-nitrophenyl phosphate | Rattus norvegicus | AMP + phosphate | - |
? | |
| ATP + H2O | 88% of activity with p-nitrophenyl phosphate | Rattus norvegicus | ADP + phosphate | - |
? | |
| casein + H2O | 3% of activity with p-nitrophenyl phosphate | Rattus norvegicus | ? | - |
? | |
| CTP + H2O | 77% of activity with p-nitrophenyl phosphate | Rattus norvegicus | CDP + phosphate | - |
? | |
| diphosphate + H2O | 12% of activity with p-nitrophenyl phosphate | Rattus norvegicus | 2 phosphate | - |
? | |
| GTP + H2O | 31% of activity with p-nitrophenyl phosphate | Rattus norvegicus | GDP + phosphate | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Rattus norvegicus | p-nitrophenol + phosphate | - |
? | |
| phenyl phosphate + H2O | 37% of activity with p-nitrophenyl phosphate | Rattus norvegicus | phenol + phosphate | - |
? | |
| phosvitin + H2O | 3% of activity with p-nitrophenyl phosphate | Rattus norvegicus | ? | - |
? | |
| trimetaphosphate + H2O | 7% of activity with p-nitrophenyl phosphate | Rattus norvegicus | ? | - |
? | |
| UTP + 2 H2O | 22% of activity with p-nitrophenyl phosphate | Rattus norvegicus | UMP + 2 phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 16300, SDS-PAGE | Rattus norvegicus |
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