Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | 10 mM, 1.8-2fold activation of ATP hydrolysis | Secale cereale x Triticum aestivum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ascorbate | 10 mM, hydrolysis of p-nitrophenyl phosphate | Secale cereale x Triticum aestivum | |
citrate | 40 mM, 30% inhibition of ATP hydrolysis of enzyme from plump seed | Secale cereale x Triticum aestivum | |
Cu2+ | 1 mM, hydrolysis of p-nitrophenyl phosphate | Secale cereale x Triticum aestivum | |
EDTA | 10 mM | Secale cereale x Triticum aestivum | |
F- | 5 mM NaF | Secale cereale x Triticum aestivum | |
FeSO4 | - |
Secale cereale x Triticum aestivum | |
Hg2+ | 1 mM, inhibition of hydrolysis of p-nitrophenyl phosphate, slight increase of ATP hydrolysis | Secale cereale x Triticum aestivum | |
molybdate | - |
Secale cereale x Triticum aestivum | |
Pb(CH3COO)2 | 10 mM, strong inhibition of enzyme from plump seed, weak inhibition of enzyme from shriveled seed, hydrolysis of p-nitrophenyl phosphate and ATP | Secale cereale x Triticum aestivum | |
phosphate | at 1 mM or above, enzyme from plump seed | Secale cereale x Triticum aestivum | |
Tartrate | 10 mM, slight inhibition of hydrolysis of p-nitrophenyl phosphate with enzyme from plump seed, slight activation of ATP hydrolysis, enzyme from plump and shriveled seed | Secale cereale x Triticum aestivum | |
Zn2+ | 1 mM, hydrolysis of p-nitrophenyl phosphate | Secale cereale x Triticum aestivum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
ATP | enzyme from plump seed | Secale cereale x Triticum aestivum | |
0.35 | - |
p-nitrophenyl phosphate | enzyme from shriveled seed | Secale cereale x Triticum aestivum | |
0.5 | - |
ATP | enzyme from shriveled seed | Secale cereale x Triticum aestivum | |
0.57 | - |
p-nitrophenyl phosphate | enzyme from plump seed | Secale cereale x Triticum aestivum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 10 mM CaCl2, slight activation of hydrolysis of ATP, 2.3fold increase of activity with p-nitrophenyl phosphate | Secale cereale x Triticum aestivum | |
Cu2+ | 1 mM CuSO4, slight activation of ATP hydrolysis | Secale cereale x Triticum aestivum | |
Mg2+ | 10 mM MgCl2, 2-3fold increase of hydrolysis of p-nitrophenyl phosphate, slight increase of hydrolysis of ATP | Secale cereale x Triticum aestivum | |
Zn2+ | 1 mM ZnSO4, 1.4-1.5fold activation of ATP hydrolysis | Secale cereale x Triticum aestivum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Secale cereale x Triticum aestivum | - |
X Triticosecale Wittmarck | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | contains 12% carbohydrate | Secale cereale x Triticum aestivum |
Purification (Comment) | Organism |
---|---|
- |
Secale cereale x Triticum aestivum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
seed | shriveled and plump | Secale cereale x Triticum aestivum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP + H2O | 19% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 33% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed | Secale cereale x Triticum aestivum | AMP + phosphate | - |
? | |
ATP + H2O | 62% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 64% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed | Secale cereale x Triticum aestivum | ADP + phosphate | - |
? | |
diphosphate + H2O | 86% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 84% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed | Secale cereale x Triticum aestivum | 2 phosphate | - |
? | |
GTP + H2O | 74% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 69% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed | Secale cereale x Triticum aestivum | GDP + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Secale cereale x Triticum aestivum | p-nitrophenol + phosphate | - |
? | |
UTP + 2 H2O | 62% of the activity with p-nitrophenyl phosphate, enzyme from plump seed. 64% of the activity with p-nitrophenyl phosphate, enzyme from shriveled seed | Secale cereale x Triticum aestivum | UMP + 2 phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | - |
hydrolysis of ATP, enzyme from plump seed | Secale cereale x Triticum aestivum |
4.8 | - |
hydrolysis of p-nitrophenyl phosphate | Secale cereale x Triticum aestivum |
5.5 | - |
hydrolysis of ATP, enzyme from shriveled seed | Secale cereale x Triticum aestivum |