Any feedback?
Literature summary for 3.1.3.2 extracted from
- Purification and characterization of acid phosphatase from cotyledons of germinating soybean seeds (1988), Arch. Biochem. Biophys., 260, 514-520.
General Stability
| General Stability | Organism |
|---|---|
| 50-60% loss of activity after two freeze-thaw cycles | Glycine max |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphate | competitive | Glycine max |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.07 | - |
p-nitrophenyl phosphate | - |
Glycine max | |
| 0.085 | - |
Na2H2P2O7 | - |
Glycine max | |
| 0.125 | - |
ATP | - |
Glycine max | |
| 0.3 | - |
Phenyl phosphate | - |
Glycine max | |
| 0.4 | - |
2-glycerophosphate | - |
Glycine max | |
| 1 | - |
bis(p-nitrophenyl)phosphate | - |
Glycine max | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53000 | - |
gel filtration | Glycine max |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Glycine max | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cotyledon | - |
Glycine max | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Glycine max |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 0°C, stable for at least 4 weeks | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-glycerophosphate + H2O | - |
Glycine max | glycerol + phosphate | - |
? | |
| ATP + H2O | - |
Glycine max | ADP + phosphate | - |
? | |
| bis(p-nitrophenyl) phosphate + H2O | - |
Glycine max | ? | - |
? | |
| Na2H2P2O7 + H2O | - |
Glycine max | ? | - |
? | |
| p-nitrophenyl phosphate + H2O | - |
Glycine max | p-nitrophenol + phosphate | - |
? | |
| phenyl phosphate + H2O | - |
Glycine max | phenol + phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 53000, SDS-PAGE | Glycine max |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Glycine max |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 68 | - |
10 min, complete loss of activity | Glycine max |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 125 | - |
2-glycerophosphate | - |
Glycine max | |
| 188 | - |
ATP | - |
Glycine max | |
| 219 | - |
Phenyl phosphate | - |
Glycine max | |
| 232 | - |
Na2H2P2O7 | - |
Glycine max | |
| 430 | - |
p-nitrophenyl phosphate | - |
Glycine max | |
| 700 | - |
bis(p-nitrophenyl) phosphate | - |
Glycine max | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Glycine max |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
