Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 0.25-10 mM, slight activation | Hordeum vulgare | |
Triton X-100 | 0.05-1.5%, slight activation | Hordeum vulgare |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | - |
Hordeum vulgare | |
diethyl dicarbonate | - |
Hordeum vulgare | |
F- | NaF | Hordeum vulgare | |
Hg2+ | - |
Hordeum vulgare | |
molybdate | - |
Hordeum vulgare | |
Pb2+ | - |
Hordeum vulgare | |
phosphate | competitive | Hordeum vulgare | |
Urea | 2.5 M, 50% inhibition | Hordeum vulgare | |
Zn2+ | - |
Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pH-dependence of Km | Hordeum vulgare | |
0.12 | - |
p-nitrophenyl phosphate | pH 4.8 | Hordeum vulgare | |
0.19 | - |
p-nitrophenyl phosphate | pH 4.2 | Hordeum vulgare | |
0.32 | - |
p-nitrophenyl phosphate | pH 4.0 | Hordeum vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Hordeum vulgare | 5737 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
2 * 38000, SDS-PAGE | Hordeum vulgare |
77600 | - |
gel filtration | Hordeum vulgare |
79000 | - |
sucrose density gradient centrifugation | Hordeum vulgare |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
multiple enzyme forms | - |
Purification (Comment) | Organism |
---|---|
major cytoplasmic isoenzyme | Hordeum vulgare |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
root | - |
Hordeum vulgare | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
8.7 | - |
- |
Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1-naphthyl phosphate + H2O | 80% of the activity with p-nitrophenyl phosphate | Hordeum vulgare | 1-naphthol + phosphate | - |
? | |
2-naphthyl phosphate + H2O | 105% of the activity with p-nitrophenyl phosphate | Hordeum vulgare | 2-naphthol + phosphate | - |
? | |
ADP + H2O | 20% of the activity with p-nitrophenyl phosphate | Hordeum vulgare | AMP + phosphate | - |
? | |
AMP + H2O | 18% of the activity with p-nitrophenyl phosphate | Hordeum vulgare | adenosine + phosphate | - |
? | |
ATP + H2O | 75% of the activity with p-nitrophenyl phosphate | Hordeum vulgare | ADP + phosphate | - |
? | |
glucose 1-phosphate + H2O | 33% of the activity with p-nitrophenyl phosphate | Hordeum vulgare | glucose + phosphate | - |
? | |
O-phospho-L-Tyr + H2O | 62% of the activity with p-nitrophenyl phosphate | Hordeum vulgare | Tyr + phosphate | - |
? | |
O-phosphoserine + H2O | 48% of the activity with p-nitrophenyl phosphate | Hordeum vulgare | Ser + phosphate | - |
? | |
p-nitrophenyl phosphate + H2O | - |
Hordeum vulgare | p-nitrophenol + phosphate | - |
? | |
phenyl phosphate + H2O | 80% of the activity with p-nitrophenyl phosphate | Hordeum vulgare | phenol + phosphate | - |
? | |
phosphothreonine + H2O | 25% of the activity with p-nitrophenyl phosphate | Hordeum vulgare | Thr + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38000, SDS-PAGE | Hordeum vulgare |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 35 | - |
Hordeum vulgare |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
half-life: 40 min | Hordeum vulgare |
50 | - |
half-life: 5 min | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | 6.8 | - |
Hordeum vulgare |